BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6022

Title: Second Metal Binding Domain of the Menkes ATPase   PubMed: 14572476

Authors: Jones, C.; Daly, N.; Cobine, P.; Craik, D.; Dameron, C.

Citation: Jones, C.; Daly, N.; Cobine, P.; Craik, D.; Dameron, C.. "Structure and Metal Binding Studies of the Second Copper Binding Domain of the Menkes ATPase"  J. Struct. Biol. 143, 209-218 (2003).

Assembly members:
Menkes ATPase subdomain2, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Menkes ATPase subdomain2: GSMAQAGEVVLKMKVEGMTC HSCTSTIEGKIGKLQGVQRI KVSLDNQEATIVYQPHLISV EEMKKQIEAMGFPAFVKKQP KYLK

Data sets:
Data typeCount
15N chemical shifts67
1H chemical shifts515

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Menkes ATPase subdomian21

Entities:

Entity 1, Menkes ATPase subdomian2 84 residues - Formula weight is not available

1   GLYSERMETALAGLNALAGLYGLUVALVAL
2   LEULYSMETLYSVALGLUGLYMETTHRCYS
3   HISSERCYSTHRSERTHRILEGLUGLYLYS
4   ILEGLYLYSLEUGLNGLYVALGLNARGILE
5   LYSVALSERLEUASPASNGLNGLUALATHR
6   ILEVALTYRGLNPROHISLEUILESERVAL
7   GLUGLUMETLYSLYSGLNILEGLUALAMET
8   GLYPHEPROALAPHEVALLYSLYSGLNPRO
9   LYSTYRLEULYS

Samples:

sample_1: Menkes ATPase subdomain2, [U-15N], 0.5 mM; potassium phosphate 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_2: Menkes ATPase subdomain2, [U-90% 15N], 0.5 mM; potassium phosphate 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 30 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
3D 15N-seperated TOCSYnot availablenot availablenot available

Software:

X-PLOR v3.851 - refinement

DYANA v1.5 - structure solution

XEASY v3.2 - data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 6129 6130
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