BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6027

Title: NMR Solution Structure of PIIIA toxin, NMR, 20 structures   PubMed: 12006587

Authors: Nielsen, K.; Watson, M.; Adams, D.; Hammarstrom, A.; Gage, P.; Hill, J.; Craik, D.; Thomas, L.; Adams, D.; Alewood, P.; Lewis, R.

Citation: Nielsen, K.; Watson, M.; Adams, D.; Hammarstrom, A.; Gage, P.; Hill, J.; Craik, D.; Thomas, L.; Adams, D.; Alewood, P.; Lewis, R.. "Solution structure of mu-conotoxin PIIIA, a preferential inhibitor of persistent tetrodotoxin-sensitive sodium channels"  J. Biol. Chem. 277, 27247-27255 (2002).

Assembly members:
PIIIA, polymer, 22 residues, 2612 Da.

Natural source:   Common Name: Conus purpurascens   Taxonomy ID: 41690   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus purpurascens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PIIIA: XRLCCGFXKSCRSRQCKXHR CX

Data sets:
  • assigned_chemical_shifts
  • homonucl_NOEs
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mu-conotoxin PIIIA1

Entities:

Entity 1, Mu-conotoxin PIIIA 22 residues - 2612 Da.

1   PCAARGLEUCYSCYSGLYPHEHYPLYSSER
2   CYSARGSERARGGLNCYSLYSHYPHISARG
3   CYSNH2

Samples:

sample_1: PIIIA 2.0 mM; H2O 95%; D2O 5%

sample_2: PIIIA 2.0 mM; D2O 100%

sample_cond_1: pH: 3.0; temperature: 298 K

sample_cond_2: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

UXNMR - collection, processing

AURELIA - processing

X-PLOR v3.8 - refinement, structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 19384 19385
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