BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6028

Title: Northeast Structural Genomics Consortium Target TT802 Ontario Center for Structural Proteomics Target Mth0803 assignment of ribosomal protein S17E

Authors: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Cort, John; Kennedy, Michael; Arrowsmith, Cheryl

Citation: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Cort, John; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum"  The BMRB entry is the only known published source for the data..

Assembly members:
ribosomal protein S17E, polymer, 62 residues, 7199 Da.

Natural source:   Common Name: methanobacterium thermoautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanothermobacter thermoautotrophicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ribosomal protein S17E: MGNIRTSFVKRIAKEMIETH PGKFTDDFDTNKKLVEEFST VSTKHLRNKIAGYITRIISQ QK

Data sets:
Data typeCount
1H chemical shifts436
13C chemical shifts264
15N chemical shifts64
coupling constants49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mth08031

Entities:

Entity 1, mth0803 62 residues - 7199 Da.

1   METGLYASNILEARGTHRSERPHEVALLYS
2   ARGILEALALYSGLUMETILEGLUTHRHIS
3   PROGLYLYSPHETHRASPASPPHEASPTHR
4   ASNLYSLYSLEUVALGLUGLUPHESERTHR
5   VALSERTHRLYSHISLEUARGASNLYSILE
6   ALAGLYTYRILETHRARGILEILESERGLN
7   GLNLYS

Samples:

sample_1: ribosomal protein S17E, [U-13C; U-15N], 1 mM; NaCL 450 mM

cond._set1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
CCTOCSY NHnot availablenot availablenot available
HCCTOCSY NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HCCHCOSYnot availablenot availablenot available
HCCHTOCSYnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
13C NOESYnot availablenot availablenot available
13,13C 4D CCNOESYnot availablenot availablenot available

Software:

SPARKY v3.95 - spectral analysis, assignemt

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAM69968
GB AAB85303
REF NP_275942 WP_010876438
SP O26894