BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6030

Title: 1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli   PubMed: 15213457

Authors: Haupt, Melina; Coles, Murray; Truffault, Vincent; Bramkamp, Marc; Altendorf, Karlheinz; Kessler, Horst

Citation: Haupt, Melina; Coles, Murray; Truffault, Vincent; Bramkamp, Marc; Altendorf, Karlheinz; Kessler, Horst. "Letter to the Editor: 1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli"  J. Biomol. NMR 29, 437-438 (2004).

Assembly members:
potassium transporting ATPase, polymer, 156 residues, 17000 Da.
ANP, non-polymer, 506.196 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
potassium transporting ATPase: MGHHHHHHHHHHSSGHGGRH NRQASEFIPAQGVDEKTLAD AAQLASLADETPEGRSIVIL AKQRFNLRERDVQSLHATFV PFTAQSRMSGINIDNRMIRK GSVDAIRRHVEANGGHFPTD VDQKVDQVARQGATPLVVVE GSRVLGVIALKDIVKG

Data sets:
Data typeCount
1H chemical shifts898
13C chemical shifts413
15N chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KdpBN1
2ANP2

Entities:

Entity 1, KdpBN 156 residues - 17000 Da.

1   METGLYHISHISHISHISHISHISHISHIS
2   HISHISSERSERGLYHISGLYGLYARGHIS
3   ASNARGGLNALASERGLUPHEILEPROALA
4   GLNGLYVALASPGLULYSTHRLEUALAASP
5   ALAALAGLNLEUALASERLEUALAASPGLU
6   THRPROGLUGLYARGSERILEVALILELEU
7   ALALYSGLNARGPHEASNLEUARGGLUARG
8   ASPVALGLNSERLEUHISALATHRPHEVAL
9   PROPHETHRALAGLNSERARGMETSERGLY
10   ILEASNILEASPASNARGMETILEARGLYS
11   GLYSERVALASPALAILEARGARGHISVAL
12   GLUALAASNGLYGLYHISPHEPROTHRASP
13   VALASPGLNLYSVALASPGLNVALALAARG
14   GLNGLYALATHRPROLEUVALVALVALGLU
15   GLYSERARGVALLEUGLYVALILEALALEU
16   LYSASPILEVALLYSGLY

Entity 2, ANP - C10 H17 N6 O12 P3 - 506.196 Da.

1   ANP

Samples:

KdpBN-ANP: Type IA potassium translocating ATPase B chain, [U-13C; U-15N], 1.0 mM; PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER0.5 – 15.0 mM

KdpBN-ANP_cond: pH: 6.0 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHKdpBN-ANPnot availableKdpBN-ANP_cond
(H)CCH-COSYKdpBN-ANPnot availableKdpBN-ANP_cond

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DMX 900 MHz

Related Database Links:

BMRB 6029
PDB
DBJ BAA35354 BAB34148 BAG76281 BAI24086 BAI29554
EMBL CAP75183 CAQ31162 CAQ89912 CAQ97541 CAR02071
GB AAB96336 AAC73791 AAG55018 AAN79255 AAZ87406
REF NP_286410 NP_308752 NP_415225 NP_752712 WP_000087925
SP A1A8W1 A7ZJ80 A7ZXV8 B1IY32 B1LLE1