BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6032

Title: Complete 1H, 13C and 15N resonance assignments of coactosin, a cytoskeletal regulatory protein   PubMed: 15756466

Authors: Hellman, Maarit

Citation: Hellman, Maarit; Paavilainen, Ville; Annila, Arto; Lappalainen, Pekka; Perttu, Permi. "Letter to the Editor: 1H, 13C and 15N resonance assignments of coactosin, a cytoskeletal regulatory protein"  J. Biomol. NMR 30, 365-366 (2004).

Assembly members:
Coactosin, polymer, 142 residues, 15944 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Coactosin: MATKIDKEACRAAYNLVRDD GSAVIWVTFRYDGATIVPGD QGADYQHFIQQCTDDVRLFA FVRFTTGDAMSKRSKFALIT WIGEDVSGLQRAKTGTDKTL VKEVVQNFAKEFVISDRKEL EEDFIRSELKKAGGANYDAQ SE

Data sets:
Data typeCount
1H chemical shifts991
13C chemical shifts646
15N chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1coactosin1

Entities:

Entity 1, coactosin 142 residues - 15944 Da.

1   METALATHRLYSILEASPLYSGLUALACYS
2   ARGALAALATYRASNLEUVALARGASPASP
3   GLYSERALAVALILETRPVALTHRPHEARG
4   TYRASPGLYALATHRILEVALPROGLYASP
5   GLNGLYALAASPTYRGLNHISPHEILEGLN
6   GLNCYSTHRASPASPVALARGLEUPHEALA
7   PHEVALARGPHETHRTHRGLYASPALAMET
8   SERLYSARGSERLYSPHEALALEUILETHR
9   TRPILEGLYGLUASPVALSERGLYLEUGLN
10   ARGALALYSTHRGLYTHRASPLYSTHRLEU
11   VALLYSGLUVALVALGLNASNPHEALALYS
12   GLUPHEVALILESERASPARGLYSGLULEU
13   GLUGLUASPPHEILEARGSERGLULEULYS
14   LYSALAGLYGLYALAASNTYRASPALAGLN
15   SERGLU

Samples:

Sample_1: Coactosin, [U-15N; U-13C], 1.0 mM; NaCl 50 mM

Sample_2: Coactosin, [U-15N], 1.2 mM; NaCl 50 mM

Cond1: pH: 6.0; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1HN-15N-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
iHNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HC(C)H-COSYnot availablenot availablenot available
H(C)CH-TOCSYnot availablenot availablenot available
(H)C(CO)NHnot availablenot availablenot available
H(C)(CO)NHnot availablenot availablenot available
NOESY-15N-HSQCnot availablenot availablenot available
NOESY-13C-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAB25396 BAB25450 BAB26668 BAC30407 BAE28680
EMBL CAJ18469
GB AAH10249 AAH11068 AAI58747 EDL92684 EDL92686
REF NP_001101922 NP_082347 XP_003495161 XP_005073148 XP_005345814
SP B0BNA5 Q9CQI6