BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6034

Title: 1H, 15N, 13C resonance assignments and 15N-1H residual dipolar couplings in 27 kDa alpha-adaptin ear-domain   PubMed: 15213459

Authors: Denisov, Alexei; Gehring, Kalle

Citation: Denisov, Alexei; Ritter, Brigitte; McPherson, Peter; Gehring, Kalle. "Letter to the Editor: 1H, 15N, 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain"  J. Biomol. NMR 29, 441-442 (2004).

Assembly members:
ear-domain of alpha-adaptin C, polymer, 238 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ear-domain of alpha-adaptin C: SEDNFARFVCKNNGVLFENQ LLQIGLKSEFRQNLGRMFIF YGNKTSTQFLNFTPTLICAD DLQTNLNLQTKPVDPTVDGG AQVQQVVNIECISDFTEAPV LNIQFRYGGTFQNVSVKLPI TLNKFFQPTEMASQDFFQRW KQLSNPQQEVQNIFKAKHPM DTEITKAKIIGFGSALLEEV DPNPANFVGAGIIHTKTTQI GCLLRLEPNLQAQMYRLTLR TSKDTVSQRLCELLSEQF

Data sets:
Data typeCount
13C chemical shifts655
15N chemical shifts207
1H chemical shifts207
residual dipolar couplings205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-ear monomer1

Entities:

Entity 1, alpha-ear monomer 238 residues - Formula weight is not available

1   SERGLUASPASNPHEALAARGPHEVALCYS
2   LYSASNASNGLYVALLEUPHEGLUASNGLN
3   LEULEUGLNILEGLYLEULYSSERGLUPHE
4   ARGGLNASNLEUGLYARGMETPHEILEPHE
5   TYRGLYASNLYSTHRSERTHRGLNPHELEU
6   ASNPHETHRPROTHRLEUILECYSALAASP
7   ASPLEUGLNTHRASNLEUASNLEUGLNTHR
8   LYSPROVALASPPROTHRVALASPGLYGLY
9   ALAGLNVALGLNGLNVALVALASNILEGLU
10   CYSILESERASPPHETHRGLUALAPROVAL
11   LEUASNILEGLNPHEARGTYRGLYGLYTHR
12   PHEGLNASNVALSERVALLYSLEUPROILE
13   THRLEUASNLYSPHEPHEGLNPROTHRGLU
14   METALASERGLNASPPHEPHEGLNARGTRP
15   LYSGLNLEUSERASNPROGLNGLNGLUVAL
16   GLNASNILEPHELYSALALYSHISPROMET
17   ASPTHRGLUILETHRLYSALALYSILEILE
18   GLYPHEGLYSERALALEULEUGLUGLUVAL
19   ASPPROASNPROALAASNPHEVALGLYALA
20   GLYILEILEHISTHRLYSTHRTHRGLNILE
21   GLYCYSLEULEUARGLEUGLUPROASNLEU
22   GLNALAGLNMETTYRARGLEUTHRLEUARG
23   THRSERLYSASPTHRVALSERGLNARGLEU
24   CYSGLULEULEUSERGLUGLNPHE

Samples:

sample_1: ear-domain of alpha-adaptin C, [U-99% 13C; U-99% 15N], 1.0 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_2: ear-domain of alpha-adaptin C, [U-99% 15N], 1.0 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_3: ear-domain of alpha-adaptin C, [U-99% 15N], 0.3 mM; sodium phosphate buffer 25 mM; NaCl 75 mM; Pf1 phage 2.5 mg/mL

Ex-cond_1: ionic strength: 0.10 M; pH: 7.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)HNnot availablenot availablenot available
3D TROSY-HNCAnot availablenot availablenot available
3D TROSY-HN(CO)CAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D 15N-HSQC-NOESYnot availablenot availablenot available
2D IPAP 15N-HSQCnot availablenot availablenot available

Software:

xwinnmr v3.1 - collection, processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAC40392 BAD32332 BAE31240
EMBL CAA33097 CAA37791
GB AAB62703 AAH10597 AAH58099 AAH81786 EDL18116
REF NP_031485 NP_112270 XP_003461349 XP_003509821 XP_003802841
SP P17427 P18484