BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6047

Title: 1H, 15N, and 13C resonance assignment of the 23 kDa organomercurial lyase MerB in the free form   PubMed: 15213467

Authors: Di Lello, Paola; Benison, Gregory; Omichinski, James; Legault, Pascale

Citation: Di Lello, Paola; Benison, Gregory; Omichinski, James; Legault, Pascale. "1H, 15N, and 13C resonance assignment of the 23 kDa organomercurial lyase MerB in its free and mercury-bound forms"  J. Biomol. NMR 29, 457-458 (2004).

Assembly members:
Organomercurial Lyase, polymer, 212 residues, 23035 Da.

Natural source:   Common Name: Escherichia Coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Organomercurial Lyase: MKLAPYILELLTSVNRTNGT ADLLVPLLRELAKGRPVSRT TLAGILDWPAERVAAVLEQA TSTEYDKDGNIIGYGLTLRE TSYVFEIDDRRLYAWCALDT LIFPALIGRTARVSSHCAAT GAPVSLTVSPSEIQAVEPAG MAVSLVLPQEAADVRQSFCC HVHFFASVPTAEDWASKHQG LEGLAIVSVHEAFGLGQEFN RHLLQTMSSRTP

Data sets:
Data typeCount
13C chemical shifts655
1H chemical shifts974
15N chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MerB1

Entities:

Entity 1, MerB 212 residues - 23035 Da.

1   METLYSLEUALAPROTYRILELEUGLULEU
2   LEUTHRSERVALASNARGTHRASNGLYTHR
3   ALAASPLEULEUVALPROLEULEUARGGLU
4   LEUALALYSGLYARGPROVALSERARGTHR
5   THRLEUALAGLYILELEUASPTRPPROALA
6   GLUARGVALALAALAVALLEUGLUGLNALA
7   THRSERTHRGLUTYRASPLYSASPGLYASN
8   ILEILEGLYTYRGLYLEUTHRLEUARGGLU
9   THRSERTYRVALPHEGLUILEASPASPARG
10   ARGLEUTYRALATRPCYSALALEUASPTHR
11   LEUILEPHEPROALALEUILEGLYARGTHR
12   ALAARGVALSERSERHISCYSALAALATHR
13   GLYALAPROVALSERLEUTHRVALSERPRO
14   SERGLUILEGLNALAVALGLUPROALAGLY
15   METALAVALSERLEUVALLEUPROGLNGLU
16   ALAALAASPVALARGGLNSERPHECYSCYS
17   HISVALHISPHEPHEALASERVALPROTHR
18   ALAGLUASPTRPALASERLYSHISGLNGLY
19   LEUGLUGLYLEUALAILEVALSERVALHIS
20   GLUALAPHEGLYLEUGLYGLNGLUPHEASN
21   ARGHISLEULEUGLNTHRMETSERSERARG
22   THRPRO

Samples:

sample_1: Organomercurial Lyase, [U-15N], 1.0 – 1.5 mM; sodium phosphate buffer10 – 10 mM; sodium chloride10 – 10 mM; DTT7.5 – 7.5 mM; EDTA1 – 1 mM

sample_2: Organomercurial Lyase, [U-95% 13C; U-90% 15N], 1.0 – 1.5 mM; sodium phosphate buffer10 – 10 mM; sodium chloride10 – 10 mM; DTT7.5 – 7.5 mM; EDTA1 – 1 mM

Ex-cond_1: pH: 7.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D [1H-15N]-HSQCnot availablenot availableEx-cond_1
2D [1H-13C]-CT-HSQCnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D(HB)CBCA(CO)NNHnot availablenot availableEx-cond_1
3D HNCOnot availablenot availableEx-cond_1
3D H(CCO)NNH-TOCSYnot availablenot availableEx-cond_1
3D C(CO)NNH-TOCSYnot availablenot availableEx-cond_1
3D HCCH-COSYnot availablenot availableEx-cond_1
3D HCCH-TOCSYnot availablenot availableEx-cond_1
2D(Hb)Cb(CgCd)Hdnot availablenot availableEx-cond_1
2D(Hb)Cb(CgCdCe)Henot availablenot availableEx-cond_1
3D 15N-edited TOCSYnot availablenot availableEx-cond_1
3D [1H-15N]-NOESY-HSQCnot availablenot availableEx-cond_1
3D [1H-13C]-HMQC-NOESYnot availablenot availableEx-cond_1
3D HNHAnot availablenot availableEx-cond_1

Software:

NMRView v5.0.3 -

PIPP -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 6055
PDB
DBJ BAA36432 BAN84179
EMBL CAC79205 CAK12695 CDI28660 CDR86370
GB AAA88369 AAB49639 AAD46510 AAD46511 AAR91470
REF NP_361074 WP_000317307 WP_000761850 WP_001326385 WP_015059358
SP P08664 P62225 P77072 Q91UN2