BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6053

Title: 1H, 13C and 15N resonance assignments of the N-terminal 16kDa domain of Escherichia coli Ada Protein   PubMed: 15213462

Authors: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu

Citation: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu. "Letter to the Editer: 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada Protein "  J. Biomol. NMR 29, 447-448 (2004).

Assembly members:
Escherichia coli Ada Protein, polymer, 146 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Escherichia coli Ada Protein: MKKATCLTDDQRWQSVLARD PNADGEFVFAVRTTGIFCRP SCRARHALRENVSFYANASE ALAAGFRPCKRCQPEKANAQ QHRLDKITHACRLLEQETPV TLEALADQVAMSPFHLHRLF KATTGMTPKAWQQAWRARRL RESLAK

Data sets:
Data typeCount
1H chemical shifts948
13C chemical shifts586
15N chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Ada16k1
2ZINC (II) ION2

Entities:

Entity 1, N-Ada16k 146 residues - Formula weight is not available

1   METLYSLYSALATHRCYSLEUTHRASPASP
2   GLNARGTRPGLNSERVALLEUALAARGASP
3   PROASNALAASPGLYGLUPHEVALPHEALA
4   VALARGTHRTHRGLYILEPHECYSARGPRO
5   SERCYSARGALAARGHISALALEUARGGLU
6   ASNVALSERPHETYRALAASNALASERGLU
7   ALALEUALAALAGLYPHEARGPROCYSLYS
8   ARGCYSGLNPROGLULYSALAASNALAGLN
9   GLNHISARGLEUASPLYSILETHRHISALA
10   CYSARGLEULEUGLUGLNGLUTHRPROVAL
11   THRLEUGLUALALEUALAASPGLNVALALA
12   METSERPROPHEHISLEUHISARGLEUPHE
13   LYSALATHRTHRGLYMETTHRPROLYSALA
14   TRPGLNGLNALATRPARGALAARGARGLEU
15   ARGGLUSERLEUALALYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Escherichia coli Ada Protein, [U-98% 13C; U-98% 15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT P06134
REF YP_001724427 NP_416717 NP_311129 NP_288793 AP_002809
GenBank ACA77100 AAG57348 AAC75273 AAA23412 AAA16408
DBJ BAB36525 BAA15996
PDB
BMRB 6054