BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6054

Title: 1H, 13C and 15N resonance assignments of the methylated N-terminal 16kDa domain of Escherichia coli Ada Protein   PubMed: 15213462

Authors: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu

Citation: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu. "Letter to the Editer: 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada Protein"  J. Biomol. NMR 29, 447-448 (2004).

Assembly members:
Escherichia coli Ada Protein, polymer, 146 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Escherichia coli Ada Protein: MKKATCLTDDQRWQSVLARD PNADGEFVFAVRTTGIFXRP SCRARHALRENVSFYANASE ALAAGFRPCKRCQPEKANAQ QHRLDKITHACRLLEQETPV TLEALADQVAMSPFHLHRLF KATTGMTPKAWQQAWRARRL RESLAK

Data sets:
Data typeCount
13C chemical shifts602
15N chemical shifts147
1H chemical shifts968

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1meC38 N-Ada16k1
2ZINC (II) ION2

Entities:

Entity 1, meC38 N-Ada16k 146 residues - Formula weight is not available

1   METLYSLYSALATHRCYSLEUTHRASPASP
2   GLNARGTRPGLNSERVALLEUALAARGASP
3   PROASNALAASPGLYGLUPHEVALPHEALA
4   VALARGTHRTHRGLYILEPHESMCARGPRO
5   SERCYSARGALAARGHISALALEUARGGLU
6   ASNVALSERPHETYRALAASNALASERGLU
7   ALALEUALAALAGLYPHEARGPROCYSLYS
8   ARGCYSGLNPROGLULYSALAASNALAGLN
9   GLNHISARGLEUASPLYSILETHRHISALA
10   CYSARGLEULEUGLUGLNGLUTHRPROVAL
11   THRLEUGLUALALEUALAASPGLNVALALA
12   METSERPROPHEHISLEUHISARGLEUPHE
13   LYSALATHRTHRGLYMETTHRPROLYSALA
14   TRPGLNGLNALATRPARGALAARGARGLEU
15   ARGGLUSERLEUALALYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Escherichia coli Ada Protein, [U-98% 13C; U-98% 15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 6605 6053
PDB
DBJ BAA15996.1 BAB36525.1
GenBank AAA16408.1 AAC75273.1 AAA23412.1 EAY49314.1 AAG57348.1
REF AP_002809.1 NP_416717.1 ZP_00736701.1 ZP_01698335.1 NP_288793.1
SWISS-PROT P06134