BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6058

Title: 1H, 13C and 15N Assignments for the Hypothetical Archaeglobus fulgidis protein AF2095. Northeast Structural Genomics Consortium target GR4   PubMed: 15452442

Authors: Powers, Robert; Acton, Tom; Huang, Janet; Liu, Jinfeng; Ma, Lichung; Montelione, Gaetano; Rost, Burkard; Xiao, Rong

Citation: Powers, Robert; Acton, Tom; Chiang, Yiwen; Rajan, P.; Cort, John; Kennedy, Michael; Liu, Jinfeng; Ma, Lichung; Rost, Burkard; Montelione, Gaetano. "Letter to the Editor: 1H, 13C and 15N Assignments for the Archaeglobus fulgidis protein AF2095. "  J. Biomol. NMR 30, 107-108 (2004).

Assembly members:
Hypothetical Archaeglobus fulgidis protein AF2095, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Archaeoglobus fulgidus   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: not available   Genus/species: Archaeoglobus fulgidus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Hypothetical Archaeglobus fulgidis protein AF2095: MTLKQVIVVRDDLKLSRGKL AVQVAHAAIIGYLKSDSSLR RKWLDEGQKKVVLKVKSLEE LLGIKHKAESLGLVTGLVQD AGLTEVPPGTITAVVIGPDE ERKIDKVTGNLPLLKLEHHH HHH

Data sets:
Data typeCount
1H chemical shifts832
13C chemical shifts495
15N chemical shifts111
coupling constants70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GR4 monomer1

Entities:

Entity 1, GR4 monomer 123 residues - Formula weight is not available

1   METTHRLEULYSGLNVALILEVALVALARG
2   ASPASPLEULYSLEUSERARGGLYLYSLEU
3   ALAVALGLNVALALAHISALAALAILEILE
4   GLYTYRLEULYSSERASPSERSERLEUARG
5   ARGLYSTRPLEUASPGLUGLYGLNLYSLYS
6   VALVALLEULYSVALLYSSERLEUGLUGLU
7   LEULEUGLYILELYSHISLYSALAGLUSER
8   LEUGLYLEUVALTHRGLYLEUVALGLNASP
9   ALAGLYLEUTHRGLUVALPROPROGLYTHR
10   ILETHRALAVALVALILEGLYPROASPGLU
11   GLUARGLYSILEASPLYSVALTHRGLYASN
12   LEUPROLEULEULYSLEUGLUHISHISHIS
13   HISHISHIS

Samples:

sample_1: Hypothetical Archaeglobus fulgidis protein AF2095, [U-99% 13C; U-99% 15N], 1.0 mM

condition_1: pH: 6.5 na; temperature: 313 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNHAnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
N15-edited NOESYnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

AutoAssign v1.11.1 - automated assignment

PIPP v4.3.5 - data analysis

NMRPipe v2.2 - data processing

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 750 MHz

Related Database Links:

PDB
GB AAB89160 AIG99082
REF NP_070920 WP_010879586
SP O28185