BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6059

Title: Ras-induced losing of interaction between C1 and RBD domains of Nore1   PubMed: 16698549

Authors: Guiberman, Elena; Wohlgemuth, Sabine; Herrmann, Christian; Harjes, Stefan; Muller, Karl-Heinz; Bayer, Peter

Citation: Harjes, Elena; Harjes, Stefan; Wohlgemuth, Sabine; Muller, Karl-Heinz; Krieger, Elmar; Herrmann, Christian; Bayer, Peter. "GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1"  Structure 14, 881-888 (2006).

Assembly members:
putative Ras effector, polymer, 59 residues, 8255 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
putative Ras effector: PRVLAERGEGHRFVELALRG GPGWCDLCGREVLRQALRCA NCKFTCHSECRSLIQLDCR

Data sets:
Data typeCount
13C chemical shifts110
1H chemical shifts329
15N chemical shifts55
coupling constants42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C1 domain, Nore11
2ZINC ION (2+), 12
3ZINC ION (2+), 22

Entities:

Entity 1, C1 domain, Nore1 59 residues - 8255 Da.

1   PROARGVALLEUALAGLUARGGLYGLUGLY
2   HISARGPHEVALGLULEUALALEUARGGLY
3   GLYPROGLYTRPCYSASPLEUCYSGLYARG
4   GLUVALLEUARGGLNALALEUARGCYSALA
5   ASNCYSLYSPHETHRCYSHISSERGLUCYS
6   ARGSERLEUILEGLNLEUASPCYSARG

Entity 2, ZINC ION (2+), 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: putative Ras effector, [U-95% 13C; U-90% 15N], 2.0 mM; pottasium phosphate puffer 20.0 mM; NaN3 0.5 mM; DTE 4 mM

condition_1: pH: 6.9; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_1not availablecondition_1
1H-15N NOESYsample_1not availablecondition_1
CCONHsample_1not availablecondition_1
HCCONHsample_1not availablecondition_1
1H-N15 TOCSYsample_1not availablecondition_1
HCCH-COSYsample_1not availablecondition_1

Software:

Aurelia v2.8.11 - Visualisation of NMR-Spectra

NMR spectrometers:

  • INOVA Varian 600 MHz

Related Database Links:

PDB
DBJ BAE33312
GB AAC08580 AAH89605 EDL39713 EDL39714
REF NP_061220
SP Q5EBH1