BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6063

Title: E6-binding zinc finger (E6apc2)   PubMed: 15182185

Authors: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.

Citation: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.. "DESIGN AND CHARACTERIZATION OF HELICAL PEPTIDES THAT INHIBIT THE E6 PROTEIN OF PAPILLOMAVIRUS"  Biochemistry 43, 7421-7431 (2004).

Assembly members:
E6apc2, polymer, 35 residues, Formula weight is not available

Natural source:   Common Name: Papillomavirus   Taxonomy ID: 10558   Superkingdom: Viruses   Kingdom: not available   Genus/species: Papillomavirus not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
E6apc2: XYKFACPECPKRFMRSDHLS KHITLHELLGEERRX

Data sets:
Data typeCount
1H chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E6apc2 peptide1

Entities:

Entity 1, E6apc2 peptide 35 residues - Formula weight is not available

1   ACETYRLYSPHEALACYSPROGLUCYSPRO
2   LYSARGPHEMETARGSERASPHISLEUSER
3   LYSHISILETHRLEUHISGLULEULEUGLY
4   GLUGLUARGARGNH2

Samples:

sample_1: E6apc2 2.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_2: E6apc2 2.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; D2O 99.96%

sample_cond_1: ionic strength: 4 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1

Software:

xwinnmr v3.5 - collection, processing

CNS v1.1 - processing, refinement, structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
REF XP_010223949