BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6069

Title: Chemical Shift Assignments for Precarnobacteriocin B2   PubMed: 15096213

Authors: Sprules, Tara; Kawulka, Karen; Gibbs, Alan; Wishart, David; Vederas, John

Citation: Sprules, Tara; Kawulka, Karen; Gibbs, Alan; Wishart, David; Vederas, John. "NMR Solution Structure of the Precursor for Carnobacteriocin B2, an Antimicrobial Peptide from Carnobacterium piscicola. "  Eur. J. Biochem. 271, 1748-1756 (2004).

Assembly members:
precarnobacteriocin B2, polymer, 64 residues, 6739 Da.

Natural source:   Common Name: Carnobacterium piscicola   Taxonomy ID: 2751   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Carnobacterium piscicola

Experimental source:   Production method: recombinant technology   Host organism: Esherichia coli

Entity Sequences (FASTA):
precarnobacteriocin B2: MNSVKELNVKEMKQLHGGVN YGNGVSCSKTKCSVNWGQAF QERYTAGINSFVSGVASGAG SIGR

Data sets:
Data typeCount
13C chemical shifts173
1H chemical shifts376
15N chemical shifts74
coupling constants49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1preCbnB21

Entities:

Entity 1, preCbnB2 64 residues - 6739 Da.

1   METASNSERVALLYSGLULEUASNVALLYS
2   GLUMETLYSGLNLEUHISGLYGLYVALASN
3   TYRGLYASNGLYVALSERCYSSERLYSTHR
4   LYSCYSSERVALASNTRPGLYGLNALAPHE
5   GLNGLUARGTYRTHRALAGLYILEASNSER
6   PHEVALSERGLYVALALASERGLYALAGLY
7   SERILEGLYARG

Samples:

sample_1: precarnobacteriocin B2, [U-15N], 1.0 mM; DSS 0.025 mM; trifluoroethanol-d3, [U-99.9% D], 30%

sample_2: precarnobacteriocin B2, [U-13C; U-15N], 1.0 mM; DSS 0.025 mM; trifluoroethanol-d3, [U-99.9% D], 30%

condition_1: pH: 2.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablecondition_1

Software:

NMRPipe v2.2 - Data processing

NMRView v5.0 - Data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT P38580
GenBank AAB81310 AAB18989 AAA72431
PDB
BMRB 4506