BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6075

Title: Backbone and side chain assignment of arsenate reductase from Bacillus Subtilis   PubMed: 15213468

Authors: Peng, Kuan; Su, Xiao-dong; Xia, Bin; Jin, Changwen

Citation: Peng, Kuan; Su, Xiao-dong; Xia, Bin; Jin, Changwen. "Letter to the Editor: 1H, 13C and 15N resonance assignments of a Bacillus Subtilis arsenate reductase "  J. Biomol. NMR 29, 459-460 (2004).

Assembly members:
Arsenate reductase, polymer, 139 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Arsenate reductase: MENKIIYFLCTGNSCRSQMA EGWAKQYLGDEWKVYSAGIE AHGLNPNAVKAMKEVGIDIS NQTSDIIDSDILNNADLVVT LCGDAADKCPMTPPHVKREH WGFDDPARAQGTEEEKWAFF QRVRDEIGNRLKEFAETGK

Data sets:
Data typeCount
1H chemical shifts859
13C chemical shifts526
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ArsC monomer1

Entities:

Entity 1, ArsC monomer 139 residues - Formula weight is not available

1   METGLUASNLYSILEILETYRPHELEUCYS
2   THRGLYASNSERCYSARGSERGLNMETALA
3   GLUGLYTRPALALYSGLNTYRLEUGLYASP
4   GLUTRPLYSVALTYRSERALAGLYILEGLU
5   ALAHISGLYLEUASNPROASNALAVALLYS
6   ALAMETLYSGLUVALGLYILEASPILESER
7   ASNGLNTHRSERASPILEILEASPSERASP
8   ILELEUASNASNALAASPLEUVALVALTHR
9   LEUCYSGLYASPALAALAASPLYSCYSPRO
10   METTHRPROPROHISVALLYSARGGLUHIS
11   TRPGLYPHEASPASPPROALAARGALAGLN
12   GLYTHRGLUGLUGLULYSTRPALAPHEPHE
13   GLNARGVALARGASPGLUILEGLYASNARG
14   LEULYSGLUPHEALAGLUTHRGLYLYS

Samples:

sample_1: Arsenate reductase, [U-13C; U-15N], 1 – 3 mM; Tris-HCl buffer 20 mM; KCl 40 mM; Na2SO4 20 mM; DTT 20 mM; Urea 20 mM; H2O 95%; D2O 5%

condition_1: pH: 6.85 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
1H-15N TOCSYHSQCnot availablenot availablenot available
1H-15N NOESYHSQCnot availablenot availablenot available
HCCHCOSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15028 6563
PDB
DBJ BAA06970 BAA12434 BAM58640
EMBL CAB14519 CEI57801 CEJ78223
GB ADV93326 AFH58698 AFH58699 AFH58701 AFH58702
REF NP_390455 WP_004398596 WP_015385204 WP_015483976 WP_029318100
SP P45947