BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6081

Title: 1H, 13C and 15N chemical shift assignment of fly troponin C   PubMed: 15213469

Authors: De Nicola, Gian Felice; Biekofsky, Rolf; Agianian, Bogos; Bullard, Belinda

Citation: De Nicola, Gian Felice; Biekofsky, Rolf; Kelly, Geoff; Agianian, Bogos; Qiu, Feng; Bullard, Belinda; Pastore, Annalisa. "Letter to the Editor: 1H, 15N and 13C Resonances of Holo Isoform 4 of Lethocerus Indicus Troponin C "  J. Biomol. NMR 29, 461-462 (2004).

Assembly members:
Troponin C, polymer, 160 residues, 17577.22 Da.

Natural source:   Common Name: Lethocerus indicus   Taxonomy ID: 212017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lethocerus indicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Troponin C: GAMGDVSKLSSNQVKLLETA FRDFETPEGSGRVSTDQIGI ILEVLGIQQTKSTIRQLIDE FDPFGNGDIDFDSFKIIGAR FLGEEVNPEQMQQELREAFR LYDKEGNGYISTDVMREILA ELDETLSSEDLDAMIDEIDA DGSGTVDFEEFMGVMTGGDE

Data sets:
Data typeCount
1H chemical shifts903
13C chemical shifts590
15N chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TnC monomer1

Entities:

Entity 1, TnC monomer 160 residues - 17577.22 Da.

1   GLYALAMETGLYASPVALSERLYSLEUSER
2   SERASNGLNVALLYSLEULEUGLUTHRALA
3   PHEARGASPPHEGLUTHRPROGLUGLYSER
4   GLYARGVALSERTHRASPGLNILEGLYILE
5   ILELEUGLUVALLEUGLYILEGLNGLNTHR
6   LYSSERTHRILEARGGLNLEUILEASPGLU
7   PHEASPPROPHEGLYASNGLYASPILEASP
8   PHEASPSERPHELYSILEILEGLYALAARG
9   PHELEUGLYGLUGLUVALASNPROGLUGLN
10   METGLNGLNGLULEUARGGLUALAPHEARG
11   LEUTYRASPLYSGLUGLYASNGLYTYRILE
12   SERTHRASPVALMETARGGLUILELEUALA
13   GLULEUASPGLUTHRLEUSERSERGLUASP
14   LEUASPALAMETILEASPGLUILEASPALA
15   ASPGLYSERGLYTHRVALASPPHEGLUGLU
16   PHEMETGLYVALMETTHRGLYGLYASPGLU

Samples:

sample_1: Troponin C, [U-15N], 0.9 mM; KCl 100 mM; CaCl(2) 9 mM

sample_2: Troponin C, [U-13C; U-15N], 0.97 mM; KCl 100 mM; CaCl(2) 9 mM

experimental_conditions: pH: 6.8; temperature: 298 K; ionic strength: 0.125 M; pressure: 997 mbar

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
15N TOCSY-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CCONHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCONHnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available

Software:

XEASY - peak assignments

NMR PIPE - spectra processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz
  • Varian UNITY 600 MHz

Related Database Links:

PDB
EMBL CAD55595