BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6082

Title: Structure in solution of SFA8, a Albumin 2S from Sunflower seeds   PubMed: 15170335

Authors: Pantoja-Uceda, David; Bruix, Marta; Shewry, Peter; Santoro, Jorge; Rico, Manuel

Citation: Pantoja-Uceda, David; Shewry, Peter; Bruix, Marta; Tatham, Arthur; Santoro, Jorge; Rico, Manuel. "Solution structure of a Methionine-Rich 2S Albumin form Sunflower Seeds: Relationship to Its Allergenic and Emulsifying Properties(,)."  Biochemistry 43, 6976-6986 (2004).

Assembly members:
methionine-rich 2S albumin from Sunflower seed, polymer, 103 residues, 12133 Da.

Natural source:   Common Name: common sunflower   Taxonomy ID: 4232   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Helianthus annuus

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
methionine-rich 2S albumin from Sunflower seed: PYGRGRTESGCYQQMEEAEM LNHCGMYLMKNLGERSQVSP RMREEDHKQLCCMQLKNLDE KCMCPAIMMMLNEPMWIRMR DQVMSMAHNLPIECNLMSQP CQM

Data sets:
Data typeCount
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SFA8 monomer1

Entities:

Entity 1, SFA8 monomer 103 residues - 12133 Da.

1   PROTYRGLYARGGLYARGTHRGLUSERGLY
2   CYSTYRGLNGLNMETGLUGLUALAGLUMET
3   LEUASNHISCYSGLYMETTYRLEUMETLYS
4   ASNLEUGLYGLUARGSERGLNVALSERPRO
5   ARGMETARGGLUGLUASPHISLYSGLNLEU
6   CYSCYSMETGLNLEULYSASNLEUASPGLU
7   LYSCYSMETCYSPROALAILEMETMETMET
8   LEUASNGLUPROMETTRPILEARGMETARG
9   ASPGLNVALMETSERMETALAHISASNLEU
10   PROILEGLUCYSASNLEUMETSERGLNPRO
11   CYSGLNMET

Samples:

sample1: methionine-rich 2S albumin from Sunflower seed 1.2 mM

Ex-cond_1: pH: 3.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available

Software:

xwinnmr -

NMR spectrometers:

  • Bruker AV 600 MHz
  • Bruker AV 800 MHz

Related Database Links:

PDB
EMBL CAA40015
SP P23110