BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6092

Title: 1H, 13C, and 15N Chemical Shift Assignments for a complex of PDZ2 from PTP-BL with the C-terminus of RIL (reversion induced LIM)   PubMed: 14725761

Authors: Walma, Tine; Vuister, Geerten

Citation: Walma, Tine; Aelen, J.; Nabuurs, S.; Oostendorp, M.; van den Berk, L.; Hendriks, W.; Vuister, Geerten. "A closed binding pocket and global destabilization modify the binding properties of an alternatively spliced form of the second PDZ domain of PTP-BL"  Structure 12, 11-20 (2004).

Assembly members:
Second PDZ domain from PTP-BL, polymer, 102 residues, 9752.07 Da.
C-terminus from RIL, polymer, 12 residues, 1301.55 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Second PDZ domain from PTP-BL: MHHHHHHMKPGDTFEVELAK TDGSLGISVTGGVNTSVRHG GIYVKAIIPKGAAESDGRIH KGDRVLAVNGVSLEGATHKQ AVETLRNTGQVVHLLLEKGQ VP
C-terminus from RIL: VAVYPNAKVELV

Data sets:
Data typeCount
13C chemical shifts278
1H chemical shifts622
15N chemical shifts105
heteronuclear NOE values82
T1 relaxation values82
T2 relaxation values82

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ2 from PTP-BL1
2RIL C-terminus2

Entities:

Entity 1, PDZ2 from PTP-BL 102 residues - 9752.07 Da.

1   METHISHISHISHISHISHISMETLYSPRO
2   GLYASPTHRPHEGLUVALGLULEUALALYS
3   THRASPGLYSERLEUGLYILESERVALTHR
4   GLYGLYVALASNTHRSERVALARGHISGLY
5   GLYILETYRVALLYSALAILEILEPROLYS
6   GLYALAALAGLUSERASPGLYARGILEHIS
7   LYSGLYASPARGVALLEUALAVALASNGLY
8   VALSERLEUGLUGLYALATHRHISLYSGLN
9   ALAVALGLUTHRLEUARGASNTHRGLYGLN
10   VALVALHISLEULEULEUGLULYSGLYGLN
11   VALPRO

Entity 2, RIL C-terminus 12 residues - 1301.55 Da.

1   VALALAVALTYRPROASNALALYSVALGLU
2   LEUVAL