BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6096

Title: The solution structure of the pH-induced monomer of dynein light chain LC8 from Drosophila   PubMed: 14767079

Authors: Makokha, M.; Huang, Y.; Montelione, G.; Edison, A.; Barbar, E.

Citation: Makokha, M.; Huang, Y.; Montelione, G.; Edison, A.; Barbar, E.. "The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila "  Protein Sci. 13, 727-734 (2004).

Assembly members:
dynein light chain, polymer, 89 residues, 10743 Da.

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
dynein light chain: MSDRKAVIKNADMSEEMQQD AVDCATQALEKYNIEKDIAA YIKKEFDKKYNPTWHCIVGR NFGSYVTHETRHFIYFYLGQ VAILLFKSG

Data sets:
Data typeCount
1H chemical shifts616
13C chemical shifts316
15N chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC8 monomer1

Entities:

Entity 1, LC8 monomer 89 residues - 10743 Da.

1   METSERASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   ALAVALASPCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   TYRILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   ARGHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Samples:

sample_1: dynein light chain, [U-15N; U-13C], 0.8 – 1.4 mM; NaCl 50 mM

sample_cond_1: pH: 3.0; temperature: 303 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-NOESYnot availablenot availablenot available
13C-NOESYnot availablenot availablenot available
H/D EXCHANGEnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
COSYnot availablenot availablenot available
CT-HSQCnot availablenot availablenot available
backbone TR experimentsnot availablenot availablenot available
3D TOCSYSnot availablenot availablenot available

Software:

FELIX v97 - processing

NMRPipe v2.1 - processing

AutoAssign v1.9 - data analysis

AutoStructure v1.1.2 - refinement

DYANA v1.5 - refinement

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 15076 15077 15078 15953 16847 17692
PDB
EMBL CDQ62741 CDQ75050
GB AAB04148 AAD00072 AAD00073 AAD00074 AAF45975
REF NP_001135068 NP_001177683 NP_001188181 NP_001245530 NP_001245836
SP O96860 Q24117
TPG DAA34424