BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6104

Title: Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha   PubMed: 15155727

Authors: Weljie, A.; Vogel, H.

Citation: Weljie, A.; Vogel, H.. "Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha"  J. Biol. Chem. 279, 35494-35502 (2004).

Assembly members:
Calcium-dependent protein kinase SK5, polymer, 188 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: soybean   Taxonomy ID: 3847   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Glycine max

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calcium-dependent protein kinase SK5: AERLSEEEIGGLKELFKMID TDNSGTITFDELKDGLKRVG SELMESEIKDLMDAADIDKS GTIDYGEFIAATVHLNKLER EENLVSAFSYFDKDGSGYIT LDEIQQACKDFGLDDIHIDD MIKEIDQDNDGQIDYGEFAA MMRKRKGNGGIGRRTMRKTL NLRDALGLVDNGSNQVIEGY FKHHHHHH

Data sets:
Data typeCount
15N chemical shifts157
1H chemical shifts891
13C chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Calcium-dependent protein kinase SK51
2CALCIUM (II) ION 12
3CALCIUM (II) ION 22
4CALCIUM (II) ION 32
5CALCIUM (II) ION 42

Entities:

Entity 1, Calcium-dependent protein kinase SK5 188 residues - 40.078 Da.

1   ALAGLUARGLEUSERGLUGLUGLUILEGLY
2   GLYLEULYSGLULEUPHELYSMETILEASP
3   THRASPASNSERGLYTHRILETHRPHEASP
4   GLULEULYSASPGLYLEULYSARGVALGLY
5   SERGLULEUMETGLUSERGLUILELYSASP
6   LEUMETASPALAALAASPILEASPLYSSER
7   GLYTHRILEASPTYRGLYGLUPHEILEALA
8   ALATHRVALHISLEUASNLYSLEUGLUARG
9   GLUGLUASNLEUVALSERALAPHESERTYR
10   PHEASPLYSASPGLYSERGLYTYRILETHR
11   LEUASPGLUILEGLNGLNALACYSLYSASP
12   PHEGLYLEUASPASPILEHISILEASPASP
13   METILELYSGLUILEASPGLNASPASNASP
14   GLYGLNILEASPTYRGLYGLUPHEALAALA
15   METMETARGLYSARGLYSGLYASNGLYGLY
16   ILEGLYARGARGTHRMETARGLYSTHRLEU
17   ASNLEUARGASPALALEUGLYLEUVALASP
18   ASNGLYSERASNGLNVALILEGLUGLYTYR
19   PHELYSHISHISHISHISHISHIS

Entity 2, CALCIUM (II) ION 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calcium-dependent protein kinase SK5, [U-15N; U-13C], 0.8 mM; unlabelled JD peptide 0.9 mM; H2-Imidazole 50 mM; NaCl 150 mM; CaCl2 10 mM; H2O 95%; D2O 5%

sample_2: Calcium-dependent protein kinase SK5, [U-15N; U-13C], 0.5 mM; unlabelled JD peptide 0.6 mM; H2-Imidazole 50 mM; NaCl 200 mM; CaCl2 10 mM; H2O 95%; D2O 5%

sample_3: Calcium-dependent protein kinase SK5, [U-15N; U-13C], 0.4 mM; unlabelled JD peptide 0.5 mM; H2-Imidazole 50 mM; NaCl 200 mM; CaCl2 10 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 7.3; temperature: 313 K; ionic strength: 260 mM; pressure: 1 atm

sample_cond_2: pH: 7.3; temperature: 313 K; ionic strength: 210 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

XWINNMR v2.6 - collection

NMRPipe v2.1 - processing

NMRView v5.04 - data analysis

CNS v1.1 - structure solution, refinement

ARIA v1.2 - iterative matrix relaxation

VNMR vunknown - collection

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
GB AAB00806 AAP03012 KHN16906 KHN17436
REF NP_001238517 NP_001241962 XP_006584637
SP P28583