BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6108

Title: Solution structure of GlgS protein from E. coli   PubMed: 15161493

Authors: Kozlov, G.; Gehring, K.

Citation: Kozlov, G.; Elias, D.; Cygler, M.; Gehring, K.. "Structure of GlgS from Escherichia coli suggests a role in protein-protein interactions"  BMC Biol. 2, 10-10 (2004).

Assembly members:
glgS, polymer, 86 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
glgS: MGSSHHHHHHSSGLVPRGSH MDHSLNSLNNFDFLARSFAR MHAEGRPVDILAVTGNMDEE HRTWFCARYAWYCQQMMQAR ELELEH

Data sets:
Data typeCount
1H chemical shifts424
13C chemical shifts188
15N chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1glgS1

Entities:

Entity 1, glgS 86 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASPHISSERLEUASNSERLEUASNASN
4   PHEASPPHELEUALAARGSERPHEALAARG
5   METHISALAGLUGLYARGPROVALASPILE
6   LEUALAVALTHRGLYASNMETASPGLUGLU
7   HISARGTHRTRPPHECYSALAARGTYRALA
8   TRPTYRCYSGLNGLNMETMETGLNALAARG
9   GLULEUGLULEUGLUHIS

Samples:

sample_1: glgS, [U-13C; U-15N], 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_2: glgS 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_3: glgS 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; D2O 100%

sample_cond_1: pH: 6.7; temperature: 298 K; ionic strength: 5 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

XWINNMR v2.1 - collection, processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

Xplor-NIH v2.9.2 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAA16577 BAB37354 BAG78852 BAI27331 BAI32446
EMBL CAA77940 CAP77523 CAQ33386 CAR00010 CAR04675
GB AAC76085 AAG58183 AAN44566 AAN82242 AAP18379
REF NP_289624 NP_311958 NP_417521 NP_708859 NP_755668
SP A7ZRT0 A8A4K4 B1IRR7 B1LF40 B1XG54