BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6109

Title: Chemical shifts of assigned proton resonances of BmKK4 at 303K and pH=3.66   PubMed: 12906891

Authors: Wu, H.; Zhang, N.; Chen, X.; Cao, C.; Wang, Y.; Li, M.; Hu, G.

Citation: Li, Minghua; Zhang, Naixia; Chen, Xiang; Wu, G.; Wu, Houming; Hu, Guoyuan. "BmKK4, a novel toxin from the venom of Asian scorpion Buthus martensi Karsch, inhibits potassium currents in rat hippocampal neurons in vitro"  Toxicon 42, 199-205 (2003).

Assembly members:
BmKK4, polymer, 30 residues, Formula weight is not available

Natural source:   Common Name: Chinese scorpion   Taxonomy ID: 34649   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus martensi

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
BmKK4: XTQCQSVRDCQQYCLTPDRC SYGTCYCKTT

Data sets:
Data typeCount
1H chemical shifts176
coupling constants16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Scorpion toxin BmKK41

Entities:

Entity 1, Scorpion toxin BmKK4 30 residues - Formula weight is not available

1   PCATHRGLNCYSGLNSERVALARGASPCYS
2   GLNGLNTYRCYSLEUTHRPROASPARGCYS
3   SERTYRGLYTHRCYSTYRCYSLYSTHRTHR

Samples:

sample_1: BmKK4 3.0 mM

sample_cond_1: pH: 3.02; temperature: 303 K; pressure: 1 atm

sample_cond_2: pH: 3.66; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

VNMR v6.1B - collection, processing

XEASY v1994 - data analysis

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAC38038
GB AAK61821 AAV59462
SP Q95NJ8