BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6110

Title: solution structure of a chimeric LEKTI-domain   PubMed: 15366933

Authors: Tidow, H.; Lauber, T.; Roesch, P.; Marx, U.

Citation: Tidow, H.; Lauber, T.; Vitzithum, K.; Sommerhoff, C.; Roesch, P.; Marx, U.. "Solution structure of a chimeric LEKTI domain reveals a chameleon sequence"  Biochemistry 43, 11238-11247 (2004).

Assembly members:
mutant of serine proteinase inhibitor LEKTI, polymer, 55 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
mutant of serine proteinase inhibitor LEKTI: KNEDQEMCHEFQAFMKNGKL FCPQDKKPIQSLDGIMFINK CATCKMILEKEAKSQ

Data sets:
Data typeCount
1H chemical shifts284
15N chemical shifts56
coupling constants15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Dom1PI1

Entities:

Entity 1, Dom1PI 55 residues - Formula weight is not available

1   LYSASNGLUASPGLNGLUMETCYSHISGLU
2   PHEGLNALAPHEMETLYSASNGLYLYSLEU
3   PHECYSPROGLNASPLYSLYSPROILEGLN
4   SERLEUASPGLYILEMETPHEILEASNLYS
5   CYSALATHRCYSLYSMETILELEUGLULYS
6   GLUALALYSSERGLN

Samples:

sample_1: mutant of serine proteinase inhibitor LEKTI 1.5 mM; potassium phosphate 10 mM

sample_2: mutant of serine proteinase inhibitor LEKTI, [U-100% 15N], 1.3 mM; potassium phosphate 10 mM

sample_cond_1: pH: 5.0; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D-TOCSYnot availablenot availablesample_cond_1
2D-COSYnot availablenot availablesample_cond_1
2D-NOESYnot availablenot availablesample_cond_1
1H,15N-HSQCnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
3D-1H,15N-TOCSY-HSQCnot availablenot availablesample_cond_1
3D-1H,15N-NOESY-HSQCnot availablenot availablesample_cond_1
3D-1H,15N,15N-HMQC-NOESY-HSQCnot availablenot availablesample_cond_1

Software:

NDEE -

NMRview -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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