BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6111

Title: 1H, 13C and 15N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures   PubMed: 15452439

Authors: Li, Congmin; Jin, Changwen

Citation: Li, Congmin; Jin, Changwen. "1H, 13C and 15N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures"  J. Biomol. NMR 30, 101-102 (2004).

Assembly members:
Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501, polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: Spruce budworm   Taxonomy ID: 7141   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Choristoneura fumiferana

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501: DGTCVNTNSQITANSQCVKS TATNCYIDNSQLVDTSICTR SQYSDANVKKSVTTDCNIDK SQVYLTTCTGSQYNGIYIRS STTTGTSISGPGCSISTCTI TRGVATPAAACKISGCSLSA M

Data sets:
Data typeCount
1H chemical shifts891
13C chemical shifts664
15N chemical shifts239

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cfAFP-501, monomer 11
2cfAFP-501, monomer 21

Entities:

Entity 1, cfAFP-501, monomer 1 121 residues - Formula weight is not available

1   ASPGLYTHRCYSVALASNTHRASNSERGLN
2   ILETHRALAASNSERGLNCYSVALLYSSER
3   THRALATHRASNCYSTYRILEASPASNSER
4   GLNLEUVALASPTHRSERILECYSTHRARG
5   SERGLNTYRSERASPALAASNVALLYSLYS
6   SERVALTHRTHRASPCYSASNILEASPLYS
7   SERGLNVALTYRLEUTHRTHRCYSTHRGLY
8   SERGLNTYRASNGLYILETYRILEARGSER
9   SERTHRTHRTHRGLYTHRSERILESERGLY
10   PROGLYCYSSERILESERTHRCYSTHRILE
11   THRARGGLYVALALATHRPROALAALAALA
12   CYSLYSILESERGLYCYSSERLEUSERALA
13   MET

Samples:

sample_1: Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501, [U-15N; U-13C], 0.25 – 0.5 mM

condition_1: pH: 5.7; temperature: 290 K

condition_2: pH: 5.7; temperature: 270 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAF86611