BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6121

Title: 1H, 15N, and 13C chemical shift assignments of CUG binding protein-CUGBP

Authors: Jun, Kyu-Yeon; Xia, Youlin; Han, Xiaogang; Timchenko, Lubov; Swanson, Maurice; Gao, Xiaolian

Citation: Jun, Kyu-Yeon; Xia, Youlin; Han, Xiaogang; Zhang, Hua; Timchenko, Lubov; Swanson, Maurice; Gao, Xiaolian. "1H, 15N, and 13C chemical shift assignments of RNA repeats binding protein - CUGBP1ab"  J. Biomol. NMR 30, 371-372 (2004).

Assembly members:
CUG-BP, polymer, 187 residues, 21100 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CUG-BP: MNGTLDHPDQPDLDAIKMFV GQVPRTWSEKDLRELFEQYG AVYEINVLRDRSQNPPQSKG CCFVTFYTRKAALEAQNALH NMKVLPGMHHPIQMKPADSE KNNAVEDRKLFIGMISKKCT ENDIRVMFSSFGQIEECRIL RGPDGLSRGCAFVTFTTRAM AQTAIKAMHQAQTMEGCSSP MVVKFAD

Data sets:
Data typeCount
13C chemical shifts748
15N chemical shifts201
1H chemical shifts1173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CUG binding protein1

Entities:

Entity 1, CUG binding protein 187 residues - 21100 Da.

1   METASNGLYTHRLEUASPHISPROASPGLN
2   PROASPLEUASPALAILELYSMETPHEVAL
3   GLYGLNVALPROARGTHRTRPSERGLULYS
4   ASPLEUARGGLULEUPHEGLUGLNTYRGLY
5   ALAVALTYRGLUILEASNVALLEUARGASP
6   ARGSERGLNASNPROPROGLNSERLYSGLY
7   CYSCYSPHEVALTHRPHETYRTHRARGLYS
8   ALAALALEUGLUALAGLNASNALALEUHIS
9   ASNMETLYSVALLEUPROGLYMETHISHIS
10   PROILEGLNMETLYSPROALAASPSERGLU
11   LYSASNASNALAVALGLUASPARGLYSLEU
12   PHEILEGLYMETILESERLYSLYSCYSTHR
13   GLUASNASPILEARGVALMETPHESERSER
14   PHEGLYGLNILEGLUGLUCYSARGILELEU
15   ARGGLYPROASPGLYLEUSERARGGLYCYS
16   ALAPHEVALTHRPHETHRTHRARGALAMET
17   ALAGLNTHRALAILELYSALAMETHISGLN
18   ALAGLNTHRMETGLUGLYCYSSERSERPRO
19   METVALVALLYSPHEALAASP

Samples:

sample_1: CUG-BP, [U-95% 13C; U-90% 15N], 0.5 mM

Ex-cond_1: pH: 5.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(CO)CACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HBHANHsample_1not availableEx-cond_1
HBHA(CO)NHsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
H(CCCO)NHsample_1not availableEx-cond_1
C(CCO)NHsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
HCCH-COSYsample_1not availableEx-cond_1
(HB)CB(CGCD)HDsample_1not availableEx-cond_1
(HB)CB(CGCDCE)HEsample_1not availableEx-cond_1
HNHAsample_1not availableEx-cond_1
Time-sharing H-CN-H NOESYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1

Software:

NMRPipe - Data processing

SPARKY - Data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

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