BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6122

Title: 1H/13C/15N assignment of the S1 domain of RNase E of E. coli

Authors: Schubert, Mario; Edge, Robert; Lario, Paula; Cook, Michael; Strynadka, Natalie; Mackie, George; McIntosh, Lawrence

Citation: Schubert, Mario; Edge, Robert; Lario, Paula; Cook, Michael; Strynadka, Natalie; Mackie, George; McIntosh, Lawrence. "Structural Characterization of the RNase E S1 domain and identification of its Oligonucleotide-binding and Dimerization Interfaces"  J. Mol. Biol. 341, 37-54 (2004).

Assembly members:
S1 Rnase E, polymer, 96 residues, 10687 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
S1 Rnase E: GSHMLEQKKANIYKGKITRI EPSLEAAFVDYGAERHGFLP LKEIAREYFPANYSAHGRPN IKDVLREGQEVIVQIDKEER GNKGAALTTFISLAGS

Data sets:
Data typeCount
1H chemical shifts636
13C chemical shifts389
15N chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S1 Rnase E1

Entities:

Entity 1, S1 Rnase E 96 residues - 10687 Da.

1   GLYSERHISMETLEUGLUGLNLYSLYSALA
2   ASNILETYRLYSGLYLYSILETHRARGILE
3   GLUPROSERLEUGLUALAALAPHEVALASP
4   TYRGLYALAGLUARGHISGLYPHELEUPRO
5   LEULYSGLUILEALAARGGLUTYRPHEPRO
6   ALAASNTYRSERALAHISGLYARGPROASN
7   ILELYSASPVALLEUARGGLUGLYGLNGLU
8   VALILEVALGLNILEASPLYSGLUGLUARG
9   GLYASNLYSGLYALAALALEUTHRTHRPHE
10   ILESERLEUALAGLYSER

Samples:

sample_1: S1 Rnase E, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
H(CCO)NH-TOCSYsample_1not availableEx-cond_1
C(CO)NH-TOCSYsample_1not availableEx-cond_1
HACANsample_1not availableEx-cond_1
CBHDsample_1not availableEx-cond_1
CBHEsample_1not availableEx-cond_1
EZ-HMQC-NH2sample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Unity 500 MHz

Related Database Links:

PDB
DBJ BAA35893 BAB34885 BAG76671 BAH62786 BAI24718
EMBL CAA38206 CAA47818 CAD08310 CAP75576 CAQ31605
GB AAA23443 AAB27352 AAC74168 AAG55830 AAL20115
PIR AC0641
REF NP_287218 NP_309489 NP_415602 NP_455679 NP_460156
SP P21513