BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6131

Title: Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H

Authors: Wu, H.; Zhang, Q.

Citation: Wu, H.; Zhang, Q.. "The comparative study on the solution structures of the oxidized boving microsomal cytochrome b5 and mutant V45H"  Protein Sci. 13, 2161-2169 (2004).

Assembly members:
Cytochrome b5 Mutant V45H, polymer, 82 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cytochrome b5 Mutant V45H: AVKYYTLEEIQKHNNSKSTW LILHYKVYDLTKFLEEHPGG EEHLREQAGGDATENFEDVG HSTDARELSKTFIIGELHPD DR

Data sets:
Data typeCount
1H chemical shifts462

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cytochrome b51
2HEM_ox2

Entities:

Entity 1, Cytochrome b5 82 residues - Formula weight is not available

1   ALAVALLYSTYRTYRTHRLEUGLUGLUILE
2   GLNLYSHISASNASNSERLYSSERTHRTRP
3   LEUILELEUHISTYRLYSVALTYRASPLEU
4   THRLYSPHELEUGLUGLUHISPROGLYGLY
5   GLUGLUHISLEUARGGLUGLNALAGLYGLY
6   ASPALATHRGLUASNPHEGLUASPVALGLY
7   HISSERTHRASPALAARGGLULEUSERLYS
8   THRPHEILEILEGLYGLULEUHISPROASP
9   ASPARG

Entity 2, HEM_ox - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome b5 Mutant V45H 3.0 mM; PROTOPORPHYRIN IX CONTAINING FE 3.0 mM; H2O 90%; D2O 10%; Phosphate buffer 25 mM

sample_2: Cytochrome b5 Mutant V45H 3.0 mM; PROTOPORPHYRIN IX CONTAINING FE 3.0 mM; D2O 100%; Phosphate buffer 25 mM

sample_cond_1: pH: 7.00; temperature: 293 K; ionic strength: 25 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

DYANA v1.5 - structure solution

VNMR v6.1B - collection

XEASY - data analysis

AMBER v6.0 - refinement

NMR spectrometers:

  • UNITY Varian 800 MHz

Related Database Links:

SWISS-PROT P00171
REF NP_776458
PRF 1803548B 1106188A
GenBank AAI08114 AAC14455
EMBL CAA31949
PDB
BMRB 5745 4810 4806 4803 294 1324 1323