BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6134

Title: THE NMR STRUCTURE OF A STABLE AND COMPACT ALL-beta-SHEET VARIANT OF INTESTINAL FATTY ACID-BINDING PROTEIN   PubMed: 15096629

Authors: Ogbay, B.; DeKoster, G.; Cistola, D.

Citation: Ogbay, B.; DeKoster, G.; Cistola, D.. "THE NMR STRUCTURE OF A STABLE AND COMPACT ALL-beta-SHEET VARIANT OF INTESTINAL FATTY ACID-BINDING PROTEIN"  Protein Sci. 13, 1227-1237 (2004).

Assembly members:
Second generation helix-less intestinal fatty acid binding protein, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Second generation helix-less intestinal fatty acid binding protein: AFDGTWKVGGLKLTITQEGN KFTVKESSNFRNIDVVFELG VDFAYSLADGTELTGTWTME GNKLVGKFKRVDNGKELIAV REISGNELIQTYTYEGVEAK RIFKKE

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts103
1H chemical shifts561

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D27-GG1

Entities:

Entity 1, D27-GG 106 residues - Formula weight is not available

1   ALAPHEASPGLYTHRTRPLYSVALGLYGLY
2   LEULYSLEUTHRILETHRGLNGLUGLYASN
3   LYSPHETHRVALLYSGLUSERSERASNPHE
4   ARGASNILEASPVALVALPHEGLULEUGLY
5   VALASPPHEALATYRSERLEUALAASPGLY
6   THRGLULEUTHRGLYTHRTRPTHRMETGLU
7   GLYASNLYSLEUVALGLYLYSPHELYSARG
8   VALASPASNGLYLYSGLULEUILEALAVAL
9   ARGGLUILESERGLYASNGLULEUILEGLN
10   THRTYRTHRTYRGLUGLYVALGLUALALYS
11   ARGILEPHELYSLYSGLU

Samples:

sample_1: Second generation helix-less intestinal fatty acid binding protein 1.8 mM; potassium phosphate 20 mM; KCl 135 mM; NaCl 10 mM; NaN3 0.5%; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 165 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
13C-edited NOESY-HSQCnot availablenot availablenot available
15N-edited NOESY-HSQCnot availablenot availablenot available
3D 15N-15N edited NOESYnot availablenot availablenot available
Aromatic 13C-NOESY-HSQCnot availablenot availablenot available

Software:

CNS v1.1 - refinement, structure solution

FELIX v2001 - data analysis

VNMR v6.1 - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

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