BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6135

Title: Solution structure of conotoxin MrVIB   PubMed: 15044438

Authors: Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.

Citation: Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.. "Structures of mu O-conotoxins from Conus marmoreus: Inhibitors of TTX-sensitive and TTX-resistant sodium channels in mammalian sensory neurons"  J. Biol. Chem. ., .-. (2004).

Assembly members:
MrVIB, polymer, 31 residues, 3404.8 Da.

Natural source:   Common Name: cone snail   Taxonomy ID: 42752   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus marmoreus

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
MrVIB: ACSKKWEYCIVPILGFVYCC PGLICGPFVCV

Data sets:
Data typeCount
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MrVIB1

Entities:

Entity 1, MrVIB 31 residues - 3404.8 Da.

1   ALACYSSERLYSLYSTRPGLUTYRCYSILE
2   VALPROILELEUGLYPHEVALTYRCYSCYS
3   PROGLYLEUILECYSGLYPROPHEVALCYS
4   VAL

Samples:

sample_1: MrVIB 1 mM; CD3CN 50%; H2O 40%; D2O 10%

sample_cond_1: pH: 3.5; temperature: 290 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.6 - collection, processing

XEASY v1.3.7 - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAO02102 BAO02216 BAO02224
GB AAB34194 AAB34916
SP Q26443