BMRB Entry 6135

Title:
Solution structure of conotoxin MrVIB
Deposition date:
2004-03-03
Original release date:
2004-06-29
Authors:
Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.
Citation:

Citation: Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.. "Structures of mu O-conotoxins from Conus marmoreus: Inhibitors of TTX-sensitive and TTX-resistant sodium channels in mammalian sensory neurons"  J. Biol. Chem. ., .-. (2004).
PubMed: 15044438

Assembly members:

Assembly members:
MrVIB, polymer, 31 residues, 3404.8 Da.

Natural source:

Natural source:   Common Name: cone snail   Taxonomy ID: 42752   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus marmoreus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MrVIB: ACSKKWEYCIVPILGFVYCC PGLICGPFVCV

Data sets:
Data typeCount
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MrVIB1

Entities:

Entity 1, MrVIB 31 residues - 3404.8 Da.

1   ALACYSSERLYSLYSTRPGLUTYRCYSILE
2   VALPROILELEUGLYPHEVALTYRCYSCYS
3   PROGLYLEUILECYSGLYPROPHEVALCYS
4   VAL

Samples:

sample_1: MrVIB 1 mM; CD3CN 50%; H2O 40%; D2O 10%

sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.6 - collection, processing

XEASY v1.3.7 - data analysis

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
GenBank AAB34194 AAB34916
SWISS-PROT Q26443