BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6150

Title: 1H, 15N and 13C resonance assignments of the Apo Sm14-M20(C62V) protein from Schistosoma mansoni

Authors: Pertinhez, Thelma; Sforca, Mauricio; Alves, Adriana; Ramos, Celso; Ho, Paulo; Tendler, Miriam; Zanchin, Nilson; Spisni, Alberto

Citation: Pertinhez, Thelma; Sforca, Mauricio; Alves, Adriana; Ramos, Celso; Ho, Paulo; Tendler, Miriam; Zanchin, Nilson; Spisni, Alberto. "Letter to the Editor: 1H, 15N and 13C resonance assignments of the Apo Sm14-M20(C62V) protein from Schistosoma mansoni"  J. Biomol. NMR 29, 553-554 (2004).

Assembly members:
FABP from Schistosoma mansoni, polymer, 133 residues, Formula weight is not available

Natural source:   Common Name: Schistosoma mansoni   Taxonomy ID: 6183   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistosoma mansoni

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
FABP from Schistosoma mansoni: MSSFLGKWKLSESHNFDAVM SKLGVSWATRQIGNTVTPTV TFTMDGDKMTMLTESTFKNL SVTFKFGEEFDEKTSDGRNV KSVVEKNSESKLTQTQVDPK NTTVIVREVDGDTMKTTVTV GDVTAIRNYKRLS

Data sets:
Data typeCount
1H chemical shifts779
13C chemical shifts525
15N chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FABP from Schistosoma mansoni1

Entities:

Entity 1, FABP from Schistosoma mansoni 133 residues - Formula weight is not available

1   METSERSERPHELEUGLYLYSTRPLYSLEU
2   SERGLUSERHISASNPHEASPALAVALMET
3   SERLYSLEUGLYVALSERTRPALATHRARG
4   GLNILEGLYASNTHRVALTHRPROTHRVAL
5   THRPHETHRMETASPGLYASPLYSMETTHR
6   METLEUTHRGLUSERTHRPHELYSASNLEU
7   SERVALTHRPHELYSPHEGLYGLUGLUPHE
8   ASPGLULYSTHRSERASPGLYARGASNVAL
9   LYSSERVALVALGLULYSASNSERGLUSER
10   LYSLEUTHRGLNTHRGLNVALASPPROLYS
11   ASNTHRTHRVALILEVALARGGLUVALASP
12   GLYASPTHRMETLYSTHRTHRVALTHRVAL
13   GLYASPVALTHRALAILEARGASNTYRLYS
14   ARGLEUSER

Samples:

sm14_sample: FABP from Schistosoma mansoni, [U-13C; U-15N], 0.7 mM; Na2H2PO4 50 mM

sm14_condition: pH: 6.3; temperature: 293 K; ionic strength: 0.125 M

Experiments:

NameSampleSample stateSample conditions
NHSQCsm14_samplenot availablesm14_condition
CHSQCsm14_samplenot availablesm14_condition
HNCAsm14_samplenot availablesm14_condition
HNCOCAsm14_samplenot availablesm14_condition
HNCACBsm14_samplenot availablesm14_condition
CBCACONHsm14_samplenot availablesm14_condition
NOESY NHSQCsm14_samplenot availablesm14_condition
TOCSY NHSQCsm14_samplenot availablesm14_condition
HCCHCOSYsm14_samplenot availablesm14_condition
HCCHTOCSYsm14_samplenot availablesm14_condition
hCCHTOCSY (ON CARBON)sm14_samplenot availablesm14_condition
hCCHNOESY (ON CARBON)sm14_samplenot availablesm14_condition

Software:

NMRpipe v5.0 - semi automated peak assignment

NMRview v5.0 - semi automated peak assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz
  • Bruker . 600 MHz

Related Database Links:

PDB
DBJ BAF62288
EMBL CCD60849
GB AAA63516 AAL15461 AAM18480 AAT39384 KGB36035
SP P29498