BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6158

Title: NMR solution structure of human Saposin C in the presence of detergent micelles   PubMed: 15713488

Authors: Hawkins, Cheryl; de Alba, Eva; Tjandra, Nico

Citation: Hawkins, Cheryl; de Alba, Eva; Tjandra, Nico. "Solution structure of human saposin C in a detergent environment"  J. Mol. Biol. 346, 1381-1392 (2005).

Assembly members:
saposin C, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
saposin C: SDVYCEVCEFLVKEVTKLID NNKTEKEILDAFDKMCSKLP KSLSEECQEVVDTYGSSILS ILLEEVSPELVCSMLHLCSG LVPR

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts77
1H chemical shifts547

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1saposin C1

Entities:

Entity 1, saposin C 84 residues - Formula weight is not available

1   SERASPVALTYRCYSGLUVALCYSGLUPHE
2   LEUVALLYSGLUVALTHRLYSLEUILEASP
3   ASNASNLYSTHRGLULYSGLUILELEUASP
4   ALAPHEASPLYSMETCYSSERLYSLEUPRO
5   LYSSERLEUSERGLUGLUCYSGLNGLUVAL
6   VALASPTHRTYRGLYSERSERILELEUSER
7   ILELEULEUGLUGLUVALSERPROGLULEU
8   VALCYSSERMETLEUHISLEUCYSSERGLY
9   LEUVALPROARG

Samples:

sample_1: saposin C, [U-100% 13C; U-100% 15N], 1.0 mM; sodium dodecyl sulfate 25 mM

condition_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
CBCACONHsample_1not availablecondition_1
CBCANHsample_1not availablecondition_1
HNCO-gesample_1not availablecondition_1
dipsi-(CO)NHsample_1not availablecondition_1
HBHACONHsample_1not availablecondition_1
H-dipsi-(CO)NHsample_1not availablecondition_1
15N-edited NOESYsample_1not availablecondition_1
15N-13C-edited NOESYsample_1not availablecondition_1
13C-13C-edited NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.5 - data acquisition

PIPP v4.2.8 - data assignment

NMRPipe v2.1 - data processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 5465 5951
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