BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6159

Title: 1H, 13C and 15N resonance assignments of Ca2+-free DdCAD-1, a cell-cell adhesion molecule

Authors: Lin, Zhi; Yang, daiwen; Huang, Eric; Siu, Chi-Hung

Citation: Lin, Zhi; Huang, Haibo; Siu, Chi-Hung; Yang, Daiwen. "Letter to the Editor: 1H, 13C and 15N resonance assignments of Ca2+-free DdCAD-1: a Ca2+-dependent cell-cell adhesion molecule"  J. Biomol. NMR 30, 375-376 (2004).

Assembly members:
DdCAD-1 monomer, polymer, 214 residues, 23852 Da.

Natural source:   Common Name: slime mold   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium Discoideum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
DdCAD-1 monomer: GSSVDANKVKFFFGKNCTGE SFEYNKGETVRFNNGDKWND KFMSCLVGSNVRCNIWEHNE IDTPTPGKFQELAQGSTNND LTSINGLSKFQVLPGAFQWA VDVKIVNKVNSTAGSYEMTI TPYQVDKVACKDGDDFVQLP IPKLTPPDSEIVSHLTVRQT HTPYDYVVNGSVYFKYSPTT GQVTVIKKDETFPKNMTVTQ DDNTSFIFNLNSEK

Data sets:
Data typeCount
1H chemical shifts1346
13C chemical shifts629
15N chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DdCAD monomer1

Entities:

Entity 1, DdCAD monomer 214 residues - 23852 Da.

1   GLYSERSERVALASPALAASNLYSVALLYS
2   PHEPHEPHEGLYLYSASNCYSTHRGLYGLU
3   SERPHEGLUTYRASNLYSGLYGLUTHRVAL
4   ARGPHEASNASNGLYASPLYSTRPASNASP
5   LYSPHEMETSERCYSLEUVALGLYSERASN
6   VALARGCYSASNILETRPGLUHISASNGLU
7   ILEASPTHRPROTHRPROGLYLYSPHEGLN
8   GLULEUALAGLNGLYSERTHRASNASNASP
9   LEUTHRSERILEASNGLYLEUSERLYSPHE
10   GLNVALLEUPROGLYALAPHEGLNTRPALA
11   VALASPVALLYSILEVALASNLYSVALASN
12   SERTHRALAGLYSERTYRGLUMETTHRILE
13   THRPROTYRGLNVALASPLYSVALALACYS
14   LYSASPGLYASPASPPHEVALGLNLEUPRO
15   ILEPROLYSLEUTHRPROPROASPSERGLU
16   ILEVALSERHISLEUTHRVALARGGLNTHR
17   HISTHRPROTYRASPTYRVALVALASNGLY
18   SERVALTYRPHELYSTYRSERPROTHRTHR
19   GLYGLNVALTHRVALILELYSLYSASPGLU
20   THRPHEPROLYSASNMETTHRVALTHRGLN
21   ASPASPASNTHRSERPHEILEPHEASNLEU
22   ASNSERGLULYS

Samples:

sample_1: DdCAD-1 monomer, [U-99% 13C; U-99% 15N], 0.7 – 0.8 mM

Ex-cond_1: pH: 6.2; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
H(CCO)NH TOCSYsample_1not availableEx-cond_1
CC(CO)NH TOCSYsample_1not availableEx-cond_1
H(CC)H TOCSYsample_1not availableEx-cond_1
H,13C-HSQCsample_1not availableEx-cond_1
H,15N-HSQCsample_1not availableEx-cond_1
3D 15N-EDITED NOESYsample_1not availableEx-cond_1
4D H-15N-13C-H NOESYsample_1not availableEx-cond_1
4D H-13C-13C-H NOESYsample_1not availableEx-cond_1

Software:

nmrview v5.0 -

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAA62645 AAC47135 AAK17205 EAL64543
REF XP_638048
SP P54657