BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6160

Title: Structural and biochemical evidence for disulfide bond heterogeneity in active forms of the somatomedin B domain of human vitronectin   PubMed: 15157085

Authors: Kamikubo, Y.; De Guzman, R.N.; Kroon, G.; Curriden, .; Neels, J.; Dawson, P.; Oldziej, S.; Jagielska, A.; Scheraga, H.A.; Loskutoff, D.J.; Dyson, H.J.

Citation: Kamikubo, Y.; De Guzman, R.; Kroon, G.; Curriden, S.; Neels, J.; Churchill, M.; Dawson, P.; Oldziej, S.; Jagielska, A.; Scheraga, H.; Loskutoff, D.; Dyson, H.. "Disulfide Bonding Arrangements in Active Forms of the Somatomedin B Domain of Human Vitronectin"  Biochemistry 43, 6519-6534 (2004).

Assembly members:
somatomedin B domain of vitronectin, polymer, 51 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
somatomedin B domain of vitronectin: DQESCKGRCTEGFNVDKKCQ CDELCSYYQSCCTDYTAECK PQVTRGDVFTM

Data sets:
Data typeCount
1H chemical shifts304
15N chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1somatomedin B domain of vitronectin1

Entities:

Entity 1, somatomedin B domain of vitronectin 51 residues - Formula weight is not available

1   ASPGLNGLUSERCYSLYSGLYARGCYSTHR
2   GLUGLYPHEASNVALASPLYSLYSCYSGLN
3   CYSASPGLULEUCYSSERTYRTYRGLNSER
4   CYSCYSTHRASPTYRTHRALAGLUCYSLYS
5   PROGLNVALTHRARGGLYASPVALPHETHR
6   MET

Samples:

sample_1: somatomedin B domain of vitronectin, [U-15N], 0.5 mM; sodium phosphate 20 mM

Conditions_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N TOCSY-HSQCsample_1not availableConditions_1
3D 15N NOESY-HSQCsample_1not availableConditions_1
2D 1QF COSYsample_1not availableConditions_1
2D NOESYsample_1not availableConditions_1
2QF COSYsample_1not availableConditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 900 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 15271
PDB
DBJ BAG35807 BAI47214
EMBL CAA26933 CAA28659 CAI29588
GB AAH05046 AAK60270 ABM84320 ABM87715 ADL14521
REF NP_000629 NP_001127063 XP_001106884 XP_001146856 XP_003912541
SP P04004