BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6162

Title: Full 1H, 13C and 15N Chemical Shift Assignments for the GII Domain of the yeast Linker Histone, Hho1p   PubMed: 15050829

Authors: Coles, Patrick; Thomas, Jean; Stott, Katherine; Stevens, Timothy; Ali, Tariq

Citation: Ali, Tariq; Coles, Patrick; Stevens, Timothy; Stott, Katherine; Thomas, Jean. "Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p"  J. Mol. Biol. 338, 139-148 (2004).

Assembly members:
Yeast linker histone globular domain II, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Yeast linker histone globular domain II: KASSPSSLTYKEMILKSMPQ LNDGKGSSRIVLKKYVKDTF SSKLKTSSNFDYLFNSAIKK CVENGELVQPKGPSGIIKLN KKKVKLST

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts89
1H chemical shifts620

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hho1p GII1

Entities:

Entity 1, Hho1p GII 88 residues - Formula weight is not available

1   LYSALASERSERPROSERSERLEUTHRTYR
2   LYSGLUMETILELEULYSSERMETPROGLN
3   LEUASNASPGLYLYSGLYSERSERARGILE
4   VALLEULYSLYSTYRVALLYSASPTHRPHE
5   SERSERLYSLEULYSTHRSERSERASNPHE
6   ASPTYRLEUPHEASNSERALAILELYSLYS
7   CYSVALGLUASNGLYGLULEUVALGLNPRO
8   LYSGLYPROSERGLYILEILELYSLEUASN
9   LYSLYSLYSVALLYSLEUSERTHR

Samples:

sample_1: Yeast linker histone globular domain II, [U-95% 13C; U-95% 15N], 1.0 mM; sodium phosphate 250 mM; EDTA 1 mM; DTT 1 mM; DSS 10 mM; D2O 99.8%

Ex-cond_1: ionic strength: 0.250 M; pH: 7.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
NOESY-15N-HSQCsample_1not availableEx-cond_1
15N-HSQC-TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
(H)CC(CO)NH-TOCSYsample_1not availableEx-cond_1
HC(C)H-TOCSYsample_1not availableEx-cond_1
NOESY-13C-HSQCsample_1not availableEx-cond_1

Software:

AZARA v2.7 - Spectrum processing

ANSIG v3.3 - Assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB EDZ68914