BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6165

Title: Solution structure of the PSI domain from the Met receptor   PubMed: 15358240

Authors: Kozlov, G.; Perreault, A.; Schrag, J.; Cygler, M.; Gehring, K.; Ekiel, I.

Citation: Kozlov, G.; Perreault, A.; Schrag, J.; Park, M.; Cygler, M.; Gehring, K.; Ekiel, I.. "Insights into function of PSI domains from structure of the Met receptor PSI domain"  Biochem. Biophys. Res. Commun. 321, 234-240 (2004).

Assembly members:
Hepatocyte growth factor receptor, polymer, 48 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Hepatocyte growth factor receptor: GSAMGCRHFQSCSQCLSAPP FVQCGWCHDKCVRSEECLSG TWTQQICL

Data sets:
Data typeCount
1H chemical shifts252
coupling constants38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1receptor1

Entities:

Entity 1, receptor 48 residues - Formula weight is not available

1   GLYSERALAMETGLYCYSARGHISPHEGLN
2   SERCYSSERGLNCYSLEUSERALAPROPRO
3   PHEVALGLNCYSGLYTRPCYSHISASPLYS
4   CYSVALARGSERGLUGLUCYSLEUSERGLY
5   THRTRPTHRGLNGLNILECYSLEU

Samples:

sample_1: Hepatocyte growth factor receptor 1 mM; phosphate buffer 50 mM; NaCl 0.15 M; H2O 90%; D2O 10%

sample_2: Hepatocyte growth factor receptor 1 mM; phosphate buffer 50 mM; NaCl 0.15 M; D2O 100%

sample_cond_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

xwinnmr v2.1 - collection, processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

X-PLOR NIH v2.9.2 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

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