BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6167

Title: Structural studies on the Ca-binding domain of human nucleobindin (CALNUC)

Authors: de Alba, Eva; Tjandra, Nico

Citation: de Alba, Eva; Tjandra, Nico. "Structural studies on the Ca-binding domain of human nucleobindin (CALNUC)"  Biochemistry ., .-..

Assembly members:
Nucleobindin, polymer, 99 residues, 12403 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Nucleobindin: LKEVWEELDGLDPNRFNPKT FFILHDINSDGVLDEQELEA LFTKELEKVYDPKNEEDDMR EMEEERLRMREHVMKNVDTN QDRLVTLEEFLASTQRKEF

Data sets:
Data typeCount
1H chemical shifts620
13C chemical shifts433
15N chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nucleobindin1
2calcium ion, 12
3calcium ion, 22

Entities:

Entity 1, Nucleobindin 99 residues - 12403 Da.

1   LEULYSGLUVALTRPGLUGLULEUASPGLY
2   LEUASPPROASNARGPHEASNPROLYSTHR
3   PHEPHEILELEUHISASPILEASNSERASP
4   GLYVALLEUASPGLUGLNGLULEUGLUALA
5   LEUPHETHRLYSGLULEUGLULYSVALTYR
6   ASPPROLYSASNGLUGLUASPASPMETARG
7   GLUMETGLUGLUGLUARGLEUARGMETARG
8   GLUHISVALMETLYSASNVALASPTHRASN
9   GLNASPARGLEUVALTHRLEUGLUGLUPHE
10   LEUALASERTHRGLNARGLYSGLUPHE

Entity 2, calcium ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Nucleobindin, [U-15N], 1 mM; CaCl2 5 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_2: Nucleobindin, [U-15N; U-13C], 1 mM; CaCl2 5 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_3: Nucleobindin, [U-15N; U-13C], 1 mM; CaCl2 5 mM; NaN3 0.1 mM; D2O 100%

sample_4: Nucleobindin, [U-15N; U-13C], 0.7 mM; CaCl2 5 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.8; temperature: 300 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablesample_cond_1
3D-15N separated-NOESYnot availablenot availablesample_cond_1
4D-15Nnot availablenot availablesample_cond_1
13C separated NOESYnot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
CBCACONHnot availablenot availablesample_cond_1
HNCACBnot availablenot availablesample_cond_1
HCCONHnot availablenot availablesample_cond_1
CCONHnot availablenot availablesample_cond_1
4D-13C,13C separated NOESYnot availablenot availablesample_cond_1
HCCH-TOCSYnot availablenot availablesample_cond_1
HCACONHnot availablenot availablesample_cond_1
13C-HSQC JMODnot availablenot availablesample_cond_1

Software:

UXNMR v2.5 - collection

XWINNMR v2.5 - data analysis

NMRPipe v2.1 - processing

PIPP v4.2.8 - data analysis

XPLOR-NIH v2.9.3 - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAB23644 BAB25383 BAC32214 BAD96525 BAE24451
EMBL CAA85285 CAH89813 CAH93225
GB AAA36383 AAA39842 AAB60431 AAH02356 AAH72554
REF NP_001068667 NP_001126897 NP_001128752 NP_001157134 NP_001231417
SP Q02818 Q02819 Q0P569 Q5R4U1 Q63083
TPG DAA19548