BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6171

Title: Solution structure of the second complement control protein (CCP) module of the GABA(B)R1a receptor, Pro-119 trans conformer   PubMed: 15304491

Authors: Blein, S.; Uhrin, D.; Smith, B.; White, J.; Barlow, P.

Citation: Blein, S.; Ginham, R.; Uhrin, D.; Smith, B.; Soares, D.; Veltel, S.; McIlhinney, R.; White, J.; Barlow, P.. "Structural analysis of the CCP modules of the GABAB receptor 1a: Only one of the two CCP modules is compactly folded"  J. Biol. Chem. 279, 48292-48306 (2004).

Assembly members:
Gamma-aminobutyric acid type B receptor, subunit 1, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: fungal yeast

Entity Sequences (FASTA):
Gamma-aminobutyric acid type B receptor, subunit 1: EAEFVRICSKSYLTLENGKV FLTGGDLPALDGARVEFRCD PDFHLVGSSRSVCSQGQWST PKPHCQVN

Data sets:
Data typeCount
1H chemical shifts459
13C chemical shifts230
15N chemical shifts72
coupling constants20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Gamma-aminobutyric acid type B receptor1

Entities:

Entity 1, Gamma-aminobutyric acid type B receptor 68 residues - Formula weight is not available

1   GLUALAGLUPHEVALARGILECYSSERLYS
2   SERTYRLEUTHRLEUGLUASNGLYLYSVAL
3   PHELEUTHRGLYGLYASPLEUPROALALEU
4   ASPGLYALAARGVALGLUPHEARGCYSASP
5   PROASPPHEHISLEUVALGLYSERSERARG
6   SERVALCYSSERGLNGLYGLNTRPSERTHR
7   PROLYSPROHISCYSGLNVALASN

Samples:

sample_1: Gamma-aminobutyric acid type B receptor, subunit 1, [U-15N; U-13C], 1 mM; deuterated sodium acetate buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4; temperature: 310 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1

Software:

VNMR v6.1 - collection

AZARA v2.6 - processing

ANSIG v3.3 - data analysis

CNS v1.0 - structure solution, refinement

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 6166
PDB
EMBL CAA71398 CAE84069 CAH40831 CAH40832 CAL91172
GB AAD19656 AAD19658 AAK69540 AAL26807 EDL84628
REF NP_112290 XP_006255941 XP_008770922
SP Q9Z0U4