BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6175

Title: 1H, 13C, and 15N assignments for the first TSR domain of F-spondin   PubMed: 16736493

Authors: Tossavainen, Helena

Citation: Paakkonen, Kimmo; Tossavainen, Helena; Permi, Perttu; Rakkolainen, Harri; Rauvala, Heikki; Raulo, Erkki; Kilpelainen, Ilkka; Guntert, Peter. "Solution structures of the first and fourth TSR domains of F-spondin"  Proteins 64, 665-672 (2006).

Assembly members:
thrombospondin type-1 repeat, polymer, 61 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
thrombospondin type-1 repeat: GSETCIYSNWSPWSACSSST CEKGKRMRQRMLKAQLDLSV PCPDTQDFQPCMGPGCSDED G

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts64
1H chemical shifts380

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1F-spondin TSR-11

Entities:

Entity 1, F-spondin TSR-1 61 residues - Formula weight is not available

1   GLYSERGLUTHRCYSILETYRSERASNTRP
2   SERPROTRPSERALACYSSERSERSERTHR
3   CYSGLULYSGLYLYSARGMETARGGLNARG
4   METLEULYSALAGLNLEUASPLEUSERVAL
5   PROCYSPROASPTHRGLNASPPHEGLNPRO
6   CYSMETGLYPROGLYCYSSERASPGLUASP
7   GLY

Samples:

sample_1: thrombospondin type-1 repeat, [U-13C; U-15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
iHNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(CO)CACBsample_1not availableEx-cond_1
CC(CO)NHsample_1not availableEx-cond_1
H(CCO)NHsample_1not availableEx-cond_1
HCCH-COSYsample_1not availableEx-cond_1

Software:

VNMR v6.1 C - processing

SPARKY v3.1 - analysis, peak picking

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB KFO85349
REF XP_009078154 XP_010142867