BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6177

Title: N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding   PubMed: 15341728

Authors: Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; V Konarev, P.; V., Petoukhov M.; Curry, S.; Matthews, S.

Citation: Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; Konarev, P.; Curry, S.; Matthews, S.. "Structure and RNA interactions of the N-terminal RRM domains of PTB"  Structure (Camb) 12, 1631-1643 (2004).

Assembly members:
polypyrimidine tract binding protein 1 domain 2, polymer, 164 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
polypyrimidine tract binding protein 1 domain 2: MHHHHHHGSAQAALQAVNSV QSGNLALAASAAAVDAGMAM AGQSPVLRIIVENLFYPVTL DVLHQIFSKFGTVLKIITFT KNNQFQALLQYADPVSAQHA KLSLDGQNIYNACCTLRIDF SKLTSLNVKYNNDKSRDYTR PDLPSGDSQPSLDQTMAAAF GLSV

Data sets:
Data typeCount
1H chemical shifts776
13C chemical shifts489
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PTB1-2 monomer1

Entities:

Entity 1, PTB1-2 monomer 164 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYSERALA
2   GLNALAALALEUGLNALAVALASNSERVAL
3   GLNSERGLYASNLEUALALEUALAALASER
4   ALAALAALAVALASPALAGLYMETALAMET
5   ALAGLYGLNSERPROVALLEUARGILEILE
6   VALGLUASNLEUPHETYRPROVALTHRLEU
7   ASPVALLEUHISGLNILEPHESERLYSPHE
8   GLYTHRVALLEULYSILEILETHRPHETHR
9   LYSASNASNGLNPHEGLNALALEULEUGLN
10   TYRALAASPPROVALSERALAGLNHISALA
11   LYSLEUSERLEUASPGLYGLNASNILETYR
12   ASNALACYSCYSTHRLEUARGILEASPPHE
13   SERLYSLEUTHRSERLEUASNVALLYSTYR
14   ASNASNASPLYSSERARGASPTYRTHRARG
15   PROASPLEUPROSERGLYASPSERGLNPRO
16   SERLEUASPGLNTHRMETALAALAALAPHE
17   GLYLEUSERVAL

Samples:

sample_1: polypyrimidine tract binding protein 1 domain 2, [U-13C; U-15N], 0.25 mM; Na phosphate 50 mM; NaCl 100 mM; DTT 10 mM; sodium azide 2 mM

sample_cond_1: pH: 6.5; temperature: 310 K; ionic strength: 0.3 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availablesample_cond_1
HN(CA)COsample_1not availablesample_cond_1
HNCACBsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HBHAsample_1not availablesample_cond_1
H(C)CH-TOCSYsample_1not availablesample_cond_1
(H)CCH-TOCSYsample_1not availablesample_cond_1
(HB)CB(CGCD)HDsample_1not availablesample_cond_1
15N-separated NOESYsample_1not availablesample_cond_1
13C-separated NOESYsample_1not availablesample_cond_1

Software:

XWINNMR v3.1 - collection

NMRPipe v1 - processing

NMRView v4.1.3 - data analysis

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 5409
PDB
DBJ BAB86943 BAC28230 BAC29560 BAC30837 BAC31665
EMBL CAA36321 CAA43056 CAA43202 CAA43203 CAA43973
GB AAC99798 AAH02397 AAH04383 AAH07472 AAH13694
REF NP_001070831 NP_001257986 NP_001269942 NP_001289713 NP_002810
SP P17225 P26599 Q00438 Q29099 Q8WN55
TPG DAA27468