BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6178

Title: N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding   PubMed: 15341728

Authors: Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; Konarev, P.; Petoukhov, M.; Curry, S.; Matthews, S.

Citation: Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; Konarev, P.; Curry, S.; Matthews, S.. "Structure and RNA interactions of the N-terminal RRM domains of PTB"  Structure (Camb) 12, 1631-1643 (2004).

Assembly members:
polypyrimidine tract binding protein 1 domain 1, polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
polypyrimidine tract binding protein 1 domain 1: MRGSHHHHHHGSGVPSRVIH IRKLPIDVTEGEVISLGLPF GKVTNLLMLKGKNQAFIEMN TEEAANTMVNYYTSVTPVLR GQPIYIQFSNHKELKTDSSP NQARA

Data sets:
Data typeCount
1H chemical shifts653
13C chemical shifts411
15N chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PTB1-1 monomer1

Entities:

Entity 1, PTB1-1 monomer 105 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERGLYVALPROSERARGVALILEHIS
3   ILEARGLYSLEUPROILEASPVALTHRGLU
4   GLYGLUVALILESERLEUGLYLEUPROPHE
5   GLYLYSVALTHRASNLEULEUMETLEULYS
6   GLYLYSASNGLNALAPHEILEGLUMETASN
7   THRGLUGLUALAALAASNTHRMETVALASN
8   TYRTYRTHRSERVALTHRPROVALLEUARG
9   GLYGLNPROILETYRILEGLNPHESERASN
10   HISLYSGLULEULYSTHRASPSERSERPRO
11   ASNGLNALAARGALA

Samples:

sample_1: polypyrimidine tract binding protein 1 domain 1 1 mM; Na phosphate 50 mM; NaCl 100 mM; sodium azide 2 mM; DTT 10 mM

sample_cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.3 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availablesample_cond_1
HNCACBsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1
HN(CA)COsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

XWINNMR v3.1 - collection, processing

AURELIA v2.8.11 - data analysis

NMRPipe v1 - processing

NMRView v4.1.3 - data analysis

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAC29560 BAD92147 BAI46909
EMBL CAA43056 CAA43973 CAA46443 CAA46444 CAA47386
GB AAC99798 AAH02397 AAH04383 AAH13694 AAP35465
REF NP_001269942 NP_002810 NP_114367 NP_114368 XP_001092088
SP P26599