BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6180

Title: Solution structure of the 15th domain of LEKTI   PubMed: 15366933

Authors: Vitzithum, Klaus; Roesch, Paul; Marx, Ute

Citation: Tidow, H.; Lauber, T.; Vitzithum, Klaus; Sommerhoff, C.; Roesch, Paul; Marx, Ute. "The Solution structure of a chimeric LEKTI domain reveals a chameleon sequence"  Biochemistry 43, 11238-11247 (2004).

Assembly members:
LEKTI Domain 15, polymer, 78 residues, 8569.6 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LEKTI Domain 15: GPDSEMCKDYRVLPRIGYLC PKDLKPVCGDDGQTYNNPCM LCHENLIRQTNTHIRSTGKC EESSTPGTTAASMPPSDE

Data sets:
Data typeCount
1H chemical shifts423
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LEKTI Domain 151

Entities:

Entity 1, LEKTI Domain 15 78 residues - 8569.6 Da.

1   GLYPROASPSERGLUMETCYSLYSASPTYR
2   ARGVALLEUPROARGILEGLYTYRLEUCYS
3   PROLYSASPLEULYSPROVALCYSGLYASP
4   ASPGLYGLNTHRTYRASNASNPROCYSMET
5   LEUCYSHISGLUASNLEUILEARGGLNTHR
6   ASNTHRHISILEARGSERTHRGLYLYSCYS
7   GLUGLUSERSERTHRPROGLYTHRTHRALA
8   ALASERMETPROPROSERASPGLU

Samples:

sample_1: LEKTI Domain 15, [U-15N], 1.6 mM

sample_2: LEKTI Domain 15 2.3 mM

sample_cond_1: pH: 6.4; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D-NOESYnot availablenot availablesample_cond_1
2D-TOCSYnot availablenot availablesample_cond_1
2D-COESYnot availablenot availablesample_cond_1
1H,15N-HSQCnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
3D-1H,1H,15-NOESY-HSQCnot availablenot availablesample_cond_1
3D-1H,1H,15N-TOCSY-HSQCnot availablenot availablesample_cond_1
3D-1H,15N,15N-HMQC-NOESY-HSQCnot availablenot availablesample_cond_1

Software:

NMRview v5.0.4 -

NDEE, Spinup Inc. -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
BMRB 6179