BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6194

Title: 1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 6.0

Authors: Tedford, Hugo; Maciejewski, Mark; King, Glenn

Citation: Tedford, Hugo; Maciejewski, Mark; King, Glenn. "1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 6.0"  . ., .-..

Assembly members:
Omega-atracotoxin-Hv1a, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: Blue Mountains Funnel-Web Spider   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Omega-atracotoxin-Hv1a: SPTCIPSGQPCPYNENCCSQ SCTFKENENGNTVKRCD

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts218
13C chemical shifts130
15N chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Omega-ACTX-Hv1a1

Entities:

Entity 1, Omega-ACTX-Hv1a 37 residues - Formula weight is not available

1   SERPROTHRCYSILEPROSERGLYGLNPRO
2   CYSPROTYRASNGLUASNCYSCYSSERGLN
3   SERCYSTHRPHELYSGLUASNGLUASNGLY
4   ASNTHRVALLYSARGCYSASP

Samples:

sample_1: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; NaCl 50 mM; sodium phosphate 20 mM

sample_2: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; D2O 100%

Ex-cond_1: pH: 6.0; temperature: 298 K; ionic strength: 0.075 M

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCnot availablenot availableEx-cond_1
3D 1H-13C HSQC-NOESYnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D HNCOnot availablenot availableEx-cond_1
3D HNCACOnot availablenot availableEx-cond_1
3D HCCH-COSYnot availablenot availableEx-cond_1
3D (H)CCOHN-TOCSYnot availablenot availableEx-cond_1
3D 1H-15N TOCSYnot availablenot availableEx-cond_1
3D HNHAnot availablenot availableEx-cond_1
3D HNHBnot availablenot availableEx-cond_1
2D 1H-1H NOESYnot availablenot availableEx-cond_1

Software:

nmrPipe - spectral processing

Xeasy - resonance assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4233 4937 5702 6192
PDB
EMBL CAI79354
GB ABP63656 ABP63658 AFN61295
SP A5A3H3 A5A3H5 P56207 P81596 P81598