BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6197

Title: 1H, 15N and 13C assigned chemical shifts for the Myosin type I SH3 domain (Myo3) from saccharomyces cerevisiae

Authors: Musi, Valeria; Birdsall, Berry; Pastore, Annalisa

Citation: Musi, Valeria; Birdsall, Berry; Pastore, Annalisa. "1H, 15N and 13C assigned chemical shifts for the Myosin type I SH3 domain (Myo3) from saccharomyces cerevisiae"  The BMRB entry is the only known published source for the data..

Assembly members:
Src Homology-3 domain, polymer, 70 residues, 7636.5 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Src Homology-3 domain: GKDPKFEAAYDFPGSGSSSE LPLKKGDIVFISRDEPSGWS LAKLLDGSKEGWVPTAYMTP YKDTRNTVPV

Data sets:
Data typeCount
1H chemical shifts459
13C chemical shifts286
15N chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain 11

Entities:

Entity 1, SH3 domain 1 70 residues - 7636.5 Da.

1   GLYLYSASPPROLYSPHEGLUALAALATYR
2   ASPPHEPROGLYSERGLYSERSERSERGLU
3   LEUPROLEULYSLYSGLYASPILEVALPHE
4   ILESERARGASPGLUPROSERGLYTRPSER
5   LEUALALYSLEULEUASPGLYSERLYSGLU
6   GLYTRPVALPROTHRALATYRMETTHRPRO
7   TYRLYSASPTHRARGASNTHRVALPROVAL

Samples:

sample_1: Src Homology-3 domain, [U-95% 13C; U-90% 15N], 0.6 mM

sample_2: Src Homology-3 domain, [U-90% 15N], 0.6 mM

sample_3: Src Homology-3 domain 0.6 mM

Ex-cond_1: pH: 6.9; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HNHBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
CBCANHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH TOCSYnot availablenot availableEx-cond_1
2D NOESYnot availablenot availableEx-cond_1
2D COSYnot availablenot availableEx-cond_1
2D TOCSYnot availablenot availableEx-cond_1

Software:

NMRpipe - processing data

Sparky - assignment

Xeasy - assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UNITY 500 MHz
  • Varian UNITY 600 MHz
  • Varian UNITY 800 MHz

Related Database Links:

PDB
DBJ GAA24601
EMBL CAA81970 CAY80962
GB AAB34124 AHY76119 EDN60039 EDV12973 EDZ71016
REF NP_012793
SP A6ZZJ1 P36006
TPG DAA09032