BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6198

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TM1816   PubMed: 15937903

Authors: Columbus, Linda; Peti, Wolfgang; Herrmann, Torsten; Etazady, Touraj; Klock, Heath; Lesley, Scott; Wuthrich, Kurt

Citation: Columbus, Linda; Peti, Wolfgang; Etezady-Esfarjani, Touraj; Herrmann, Torsten; Wuthrich, Kurt. "NMR structure determination of the conserved hypothetical protein TM1816 from Thermotoga maritima"  Proteins 60, 552-557 (2005).

Assembly members:
TM1816, polymer, 124 residues, 12810 Da.

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
TM1816: MIIAIPVSENRGKDSPISEH FGRAPYFAFVKVKNNAIADI SVEENPLAQDHVHGAVPNFV KEKGAELVIVRGIGRRAIAA FEAMGVKVIKGASGTVEEVV NQYLSGQLKDSDYEVHDHHH HEHH

Data sets:
Data typeCount
1H chemical shifts716
13C chemical shifts461
15N chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TM1816 polypeptide1

Entities:

Entity 1, TM1816 polypeptide 124 residues - 12810 Da.

1   METILEILEALAILEPROVALSERGLUASN
2   ARGGLYLYSASPSERPROILESERGLUHIS
3   PHEGLYARGALAPROTYRPHEALAPHEVAL
4   LYSVALLYSASNASNALAILEALAASPILE
5   SERVALGLUGLUASNPROLEUALAGLNASP
6   HISVALHISGLYALAVALPROASNPHEVAL
7   LYSGLULYSGLYALAGLULEUVALILEVAL
8   ARGGLYILEGLYARGARGALAILEALAALA
9   PHEGLUALAMETGLYVALLYSVALILELYS
10   GLYALASERGLYTHRVALGLUGLUVALVAL
11   ASNGLNTYRLEUSERGLYGLNLEULYSASP
12   SERASPTYRGLUVALHISASPHISHISHIS
13   HISGLUHISHIS

Samples:

sample_1: TM1816, [U-15N], 4.0 mM; Na phosphate 20 mM

sample_2: TM1816, [U-13C; U-15N], 2.1 mM; Na phosphate 20 mM

sample_cond_1: pH: 6; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
GB AAD36879 ADA67099 AGL50750 AHD18291
REF NP_229613 WP_004082362 YP_003346513 YP_007978175 YP_008991085