BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6200

Title: Assignment of the minicollagen 1 cysteine rich domain   PubMed: 15225618

Authors: Meier, Sebastian; Grzesiek, Stephan

Citation: Meier, Sebastian; Haussinger, Daniel; Pokidysheva, Elena; Bachinger, Hans; Grzesiek, Stephan. "Determination of a high-precision NMR structure of the minicollagen cysteine rich domain from Hydra and characterization of its disulfide bond formation"  FEBS Lett. 569, 112-116 (2004).

Assembly members:
MCRD, polymer, 24 residues, Formula weight is not available

Natural source:   Common Name: Hydra   Taxonomy ID: 6083   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hydra not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
MCRD: PCPPVCVAQCVPTCPQYCCP AKRK

Data sets:
Data typeCount
13C chemical shifts83
1H chemical shifts161
15N chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MCRD_11

Entities:

Entity 1, MCRD_1 24 residues - Formula weight is not available

1   PROCYSPROPROVALCYSVALALAGLNCYS
2   VALPROTHRCYSPROGLNTYRCYSCYSPRO
3   ALALYSARGLYS

Samples:

sample_1: MCRD 5 mM

Ex-cond_1: pH: 6.5; temperature: 288 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
homonuclear NOESYsample_1not availableEx-cond_1
TOCSYsample_1not availableEx-cond_1
nat. abundance 13C-HMQCsample_1not availableEx-cond_1
13C-HMBCsample_1not availableEx-cond_1
15N-HSQCsample_1not availableEx-cond_1

Software:

nmrpipe -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA43379
PIR A41132
REF XP_002169870