BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6201

Title: Sequence specific 1H and 15N resonance assignment of domain 1 of rat CD2 with the designed calcium binding site   PubMed: 15713084

Authors: Yang, Wei; Yang, Jenny

Citation: Yang, Wei; Wilkins, A.; Ye, Y.; Liu, Z.; Li, S.; Urbauer, J.; Hellinga, H.; Kearney, A.; van der Merwe, P.; Yang, J.. "Design of a Calcium-Binding Protein with Desired Structure in a Cell Adhesion Molecule"  J. Am. Chem. Soc. 127, 2085-2093 (2005).

Assembly members:
Ca.CD2, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ca.CD2: RDSGTVWGALGHGIDLDIPN FQMTDDIDEVRWERGSTLVA EFKRKMKPFLKSGAFEILAN GDLKIKNLTRDDSGTYNVTV YSTNGTRILNKALDLRILE

Data sets:
Data typeCount
15N chemical shifts202
1H chemical shifts1072

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD2 with designed calcium binding site1

Entities:

Entity 1, CD2 with designed calcium binding site 99 residues - Formula weight is not available

1   ARGASPSERGLYTHRVALTRPGLYALALEU
2   GLYHISGLYILEASPLEUASPILEPROASN
3   PHEGLNMETTHRASPASPILEASPGLUVAL
4   ARGTRPGLUARGGLYSERTHRLEUVALALA
5   GLUPHELYSARGLYSMETLYSPROPHELEU
6   LYSSERGLYALAPHEGLUILELEUALAASN
7   GLYASPLEULYSILELYSASNLEUTHRARG
8   ASPASPSERGLYTHRTYRASNVALTHRVAL
9   TYRSERTHRASNGLYTHRARGILELEUASN
10   LYSALALEUASPLEUARGILELEUGLU

Samples:

sample_1: Ca.CD2, [U-95% 15N], 1.0 mM; KCl 130 mM; CaCl2 10 mM; PIPES 20 mM

sample_2: Ca.CD2, [U-95% 15N], 1.0 mM; KCl 130 mM; EGTA 3 mM; PIPES 20 mM

Conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableConditions_1

Software:

SPARKY -

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4109
PDB
EMBL CAA28757
GB AAH88164 ADI96088 EDL85525
REF NP_036962
SP P08921