BMRB Entry 6266

Title:
Solution structure of Cu(I) HAH1
Deposition date:
2004-09-03
Original release date:
2005-02-08
Authors:
Anastassopoulou, I.; Banci, L.; Bertini, I.; Cantini, F.; Katsari, E.; Rosato, A.
Citation:

Citation: Anastassopoulou, I.; Banci, L.; Bertini, I.; Cantini, F.; Katsari, E.; Rosato, A.. "Solution structure of the apo and copper(I)-loaded human metallochaperone HAH1"  Biochemistry 43, 13046-13053 (2004).
PubMed: 15476398

Assembly members:

Assembly members:
Copper transport protein ATOX1, polymer, 68 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET20B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Copper transport protein ATOX1: MPKHEFSVDMTCGGCAEAVS RVLNKLGGVKYDIDLPNKKV CIESEHSMDTLLATLKKTGK TVSYLGLE

Data sets:
Data typeCount
1H chemical shifts420
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Omega-conotoxin MVIIa1
2COPPER (I) ION2

Entities:

Entity 1, Omega-conotoxin MVIIa 68 residues - Formula weight is not available

1   METPROLYSHISGLUPHESERVALASPMET
2   THRCYSGLYGLYCYSALAGLUALAVALSER
3   ARGVALLEUASNLYSLEUGLYGLYVALLYS
4   TYRASPILEASPLEUPROASNLYSLYSVAL
5   CYSILEGLUSERGLUHISSERMETASPTHR
6   LEULEUALATHRLEULYSLYSTHRGLYLYS
7   THRVALSERTYRLEUGLYLEUGLU

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: Copper transport protein ATOX1, [U-15N], 1.0 mM; sodium acetate 10 mM; H2O 90%; D2O 10%

sample_2: Copper transport protein ATOX1, [U-95% 13C; U-98% 15N], 1.0 mM; DTT 4 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

sample_cond_2: pH: 7; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
CBCANHnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
(H)CCH TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

XWINNMR - collection

XEASY v1.3 - data analysis

DIANA v1.5 - structure solution

CYANA v1.0 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 15690 18299
PDB
DBJ BAF85752
EMBL CAG33182
GB AAC51227 AAI12249 AAI12251 AAN84554 AAP88788
REF NP_004036 XP_001169183 XP_002816151 XP_003276598 XP_003829035
SP O00244
AlphaFold O00244

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks