BMRB Entry 6295

Title:
NMR Structure of the Flavin Domain from Soluble Methane Monooxygenase Reductase from Methylococcus capsulatus (Bath)
Deposition date:
2004-08-25
Original release date:
2004-09-10
Authors:
Chatwood, Lisa; Mueller, Jens; Gross, John; Wagner, Gerhard; Lippard, Stephen
Citation:

Citation: Chatwood, Lisa; Mueller, Jens; Gross, John; Wagner, Gerhard; Lippard, Stephen. "NMR structure of the flavin domain from soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)"  Biochemistry 43, 11983-11991 (2004).
PubMed: 15379538

Assembly members:

Assembly members:
Flavin domain of methane monooxygenase reductase, polymer, 252 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Methylococcus capsulatus   Taxonomy ID: 414   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Methylococcus capsulatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFAD21

Data sets:
Data typeCount
1H chemical shifts1120
15N chemical shifts247
13C chemical shifts935

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MMOR-FAD1

Entities:

Entity 1, MMOR-FAD 252 residues - Formula weight is not available

1   METCYSARGILESERPHEGLYGLUVALGLY
2   SERPHEGLUALAGLUVALVALGLYLEUASN
3   TRPVALSERSERASNTHRVALGLNPHELEU
4   LEUGLNLYSARGPROASPGLUCYSGLYASN
5   ARGGLYVALLYSPHEGLUPROGLYGLNPHE
6   METASPLEUTHRILEPROGLYTHRASPVAL
7   SERARGSERTYRSERPROALAASNLEUPRO
8   ASNPROGLUGLYARGLEUGLUPHELEUILE
9   ARGVALLEUPROGLUGLYARGPHESERASP
10   TYRLEUARGASNASPALAARGVALGLYGLN
11   VALLEUSERVALLYSGLYPROLEUGLYVAL
12   PHEGLYLEULYSGLUARGGLYMETALAPRO
13   ARGTYRPHEVALALAGLYGLYTHRGLYLEU
14   ALAPROVALVALSERMETVALARGGLNMET
15   GLNGLUTRPTHRALAPROASNGLUTHRARG
16   ILETYRPHEGLYVALASNTHRGLUPROGLU
17   LEUPHETYRILEASPGLULEULYSSERLEU
18   GLUARGSERMETARGASNLEUTHRVALLYS
19   ALACYSVALTRPHISPROSERGLYASPTRP
20   GLUGLYGLUGLNGLYSERPROILEASPALA
21   LEUARGGLUASPLEUGLUSERSERASPALA
22   ASNPROASPILETYRLEUCYSGLYPROPRO
23   GLYMETILEASPALAALACYSGLULEUVAL
24   ARGSERARGGLYILEPROGLYGLUGLNVAL
25   PHEPHEGLULYSPHELEUPROSERGLYALA
26   ALAFDA

Samples:

Sample_1: Flavin domain of methane monooxygenase reductase, [U-13C; U-15N; U-2H], 0.6 – 1.0 mM; phosphate buffer 50 mM; DTT 1 mM; NaN3 0.1%; Pefabloc protease inhibitor 2 mM; NADH 3 mM; Na2S2O4 30 mM

cond_1: pH: 7.0; temperature: 298 K; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
2D NOESYSample_1not availablecond_1
13C HMQC-NOESYSample_1not availablecond_1
13C NOESY-HSQCSample_1not availablecond_1
15N NOESY-HSQCSample_1not availablecond_1
15N TOCSY-HSQCSample_1not availablecond_1
HC(CO)NH-TOCSYSample_1not availablecond_1
H(CCO)NH-TOCSYSample_1not availablecond_1
HN(COCA)CB-TROSYSample_1not availablecond_1
HN(CA)CB-TROSYSample_1not availablecond_1
HN(CA)CO-TROSYSample_1not availablecond_1
HNCO-TROSYSample_1not availablecond_1
HN(CO)CA-TROSYSample_1not availablecond_1
HNCA-TROSYSample_1not availablecond_1

Software:

FELIX v97.0 - processing

XEASY v1.3.13 - data analysis

PROSA v6.0 - processing

DYANA v1.5 - structure solution

XPLOR v3.84 - refinement

XWINNMR v2.5 - collection

VNMR v5.1a - collection

NMR spectrometers:

  • Varian UNITY-INOVA 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
GB AAB62391 AAU92722
REF WP_010960487 YP_113665
SP P22868
AlphaFold P22868

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks