BMRB Entry 6501

Title:
Backbone 1H, 15N, 13Ca and sidechain 13Cb chemical shift assignments of SARS-CoV Nsp9
Deposition date:
2005-02-12
Original release date:
2005-03-17
Authors:
Hu, Yunfei; Lescop, Ewen; Jin, Changwen
Citation:

Citation: Hu, Yunfei; Lescop, Ewen; Jin, Changwen. "Backbone dynamics of SARS-CoV Nsp9 by NMR spectroscopy"  .

Assembly members:

Assembly members:
Nsp9, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: Not applicable   Genus/species: coronavirus Severe Acute Respiratory Syndrome associated coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts189
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp9, chain 11
2Nsp9, chain 21

Entities:

Entity 1, Nsp9, chain 1 113 residues - Formula weight is not available

1   ASNASNGLULEUSERPROVALALALEUARG
2   GLNMETSERCYSALAALAGLYTHRTHRGLN
3   THRALACYSTHRASPASPASNALALEUALA
4   TYRTYRASNASNSERLYSGLYGLYARGPHE
5   VALLEUALALEULEUSERASPHISGLNASP
6   LEULYSTRPALAARGPHEPROLYSSERASP
7   GLYTHRGLYTHRILETYRTHRGLULEUGLU
8   PROPROCYSARGPHEVALTHRASPTHRPRO
9   LYSGLYPROLYSVALLYSTYRLEUTYRPHE
10   ILELYSGLYLEUASNASNLEUASNARGGLY
11   METVALLEUGLYSERLEUALAALATHRVAL
12   ARGLEUGLN

Samples:

sample_1: Nsp9, chain 1, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM; Nsp9, chain 2, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CO)CACBsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828867 YP_003858583
SP P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9
AlphaFold P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks