BMRB Entry 6530

Title:
CC45, An Artificial WW Domain Designed Using Statistical Coupling Analysis
Deposition date:
2005-03-09
Original release date:
2005-10-26
Authors:
Socolich, M.; Lockless, S.; Russ, W.; Lee, H.; Gardner, K.; Ranganathan, R.
Citation:

Citation: Socolich, M.; Lockless, S.; Russ, W.; Lee, H.; Gardner, K.; Ranganathan, R.. "Evolutionary information for specifying a protein fold"  Nature 437, 512-518 (2005).
PubMed: 16177782

Assembly members:

Assembly members:
CC45, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CC45: GSHGRSMPLPPGWERRTDVE GKVYYFNVRTLTTTWERPTI ILE

Data sets:
Data typeCount
15N chemical shifts35
1H chemical shifts269

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CC451

Entities:

Entity 1, CC45 43 residues - Formula weight is not available

1   GLYSERHISGLYARGSERMETPROLEUPRO
2   PROGLYTRPGLUARGARGTHRASPVALGLU
3   GLYLYSVALTYRTYRPHEASNVALARGTHR
4   LEUTHRTHRTHRTRPGLUARGPROTHRILE
5   ILELEUGLU

Samples:

sample_1: CC45 600 uM; sodium phosphate buffer 100 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: CC45 600 uM; sodium phosphate buffer 100 mM; NaCl 100 mM; D2O 99%

sample_3: CC45, [U-15N], 850 uM; sodium phosphate buffer 100 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available

Software:

NMRPipe v2.3 - processing

NMRView v5.2.2 - data analysis

CNS v1.1 - structure solution

ARIA v1.2 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks