BMRB Entry 6820

Title:
Solution Structure Of The Calcium-loaded N-Terminal Sensor Domain Of Centrin
Deposition date:
2005-09-09
Original release date:
2006-12-28
Authors:
Hu, H.; Fagan, P.; Bunick, C.; Sheehan, J.; Chazin, W.
Citation:

Citation: Hu, H.; Fagan, P.; Bunick, C.; Sheehan, J.; Chazin, W.. "Structure of the N-terminal calcium sensor domain of centrin reveals the biochemical basis for domain-specific function."  J. Biol. Chem. 281, 2876-2881 (2006).
PubMed: 16317001

Assembly members:

Assembly members:
Caltractin, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chlamydomonas reinhardtii   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts93
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Caltractin1

Entities:

Entity 1, Caltractin 96 residues - Formula weight is not available

1   GLYSERMETSERTYRLYSALALYSTHRVAL
2   VALSERALAARGARGASPGLNLYSLYSGLY
3   ARGVALGLYLEUTHRGLUGLUGLNLYSGLN
4   GLUILEARGGLUALAPHEASPLEUPHEASP
5   THRASPGLYSERGLYTHRILEASPALALYS
6   GLULEULYSVALALAMETARGALALEUGLY
7   PHEGLUPROLYSLYSGLUGLUILELYSLYS
8   METILESERGLUILEASPLYSASPGLYSER
9   GLYTHRILEASPPHEGLUGLUPHELEUTHR
10   METMETTHRALALYSMET

Samples:

sample_1: Caltractin1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_2: Caltractin, [U-15N], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_3: Caltractin, [U-13C], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_4: Caltractin, [U-13C; U-15N], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_5: Caltractin, [U-10% 13C], 1 – 2 mM; Tris-d11 25 mM; CaCl25 – 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 7.5 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 15N-NOESY-HSQCnot availablenot availablenot available
3D 13C-NOESY-HSQCnot availablenot availablenot available
4D 13C-HMQC-NOESY-HMQCnot availablenot availablenot available

Software:

FELIX v2000 - processing

SANE v1 - processing

DYANA v1.5 - structure solution

AMBER v7.0 - refinement

xwinnmr v2.6 - collection

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 6918
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks